Background: Considering the economic loss by columnaris disease in fish, a rapid, simpleto-
use and visual detection technology for the detection of Flavobacterium columnare (F. columnare) is
Methods: This assay utilizes LAMP for F. columnare-specific target gene amplification, and the resulting
LAMP amplicons were further analyzed by Immunochromatographic Strip (ICS) for the rapid visual
detection of F. columnare. The performance of such method includeing specificity, sensitivity,
repeatability and practical use were validated.
Results: This assay demonstrated high specificity, repeatability and has a limit of detection of 2.5 × 102
F. columnare CFU. The reliability of this assay was further confirmed by the equencing and alignment
of LAMP amplicon with the DNA sequence of F. columnare. The whole detection process was less
than 50 min.
Conclusion: The LAMP-ICS assay presented here is simple, rapid, and reliable. Moreover, it could be
easily adapted to detect other fish pathogens, and it may be more suitable for future use in the resourcelimit
region for the detection of fish pathogen on site.