Background: Muehlenbeckia sagittifolia (Ortega) Meisn. is widely used to manage
arthritis, inflammation, hepatic disorders, skin conditions, allergies, and diabetes mellitus.
Objective: The aim of this work was to investigate the antioxidant properties as well antinociceptive
activity of methanol extract of M. sagittifolia (MEMS).
Method: The antioxidant properties of methanol extract of MEMS were evaluated using different
in vitro systems. A xanthine/xanthine oxidase system, which generates superoxide
radicals, was used to evaluate the superoxide radical scavenging activity of MEMS and its
ability to inhibit xanthine oxidase activity. The interaction with peroxyl radicals was assayed
by the luminol chemiluminescence intensity induced by an azo initiator, with the
measurements of total reactive antioxidant potential and total antioxidant reactivity. The hydrogen
peroxide scavenging and metal chelating activities also were evaluated. In addition,
antinociceptive activity of MEMS was investigated by formalin test.
Results: The GC-MS analysis of the extract showed a mixture of different compounds including
hexadecanoic and octadecatrienoic acids as well as vitamin E. MEMS inhibited xanthine
oxidase activity and showed the ability to scavenge peroxyl and superoxide radicals. However,
MEMS did not show any metal chelating activity and was unable to scavenge hydrogen peroxide.
MEMS (intraperitoneally) was ineffective in first phase of the formalin test what could
exclude centrally mediated antinociceptive activity. MEMS significantly inhibited the late nociception
phase induced by formalin in mice, indicating an anti-inflammatory activity.
Conclusion: Our results suggest that Muehlenbeckia sagittifolia exhibits antioxidant activity
and antinociceptive action in inflammatory pain and can validate its traditional use in inflammatory