Background: Frequent recreational use of Anabolic Androgenic Steroids (AAS) is an instance
of substance abuse which mimics the status of a natural hormone and upon prolonged exposure
may lead to adverse drug reactions. These adverse drug reactions proceed in a manner so as to alter
the normal metabolism of an enzyme mediated pathway such as the Cytochrome P450 (CYP) family
Objective: The present study was conducted to investigate the impact of overuse of Nandrolone Decanoate
(ND), an AAS, upon CYP enzyme activity and a CYP gene, belonging to CYP1 family.
Methods: The study was carried out using normal and ND treated male albino mice. Genetic analysis
was conducted using normalized and treated cDNA and reverse transcriptase polymerase chain reaction
based assays. For enzyme assay, 0.1ml of 25 mg ND was administered to the animals twice a
week for a period of 90 days. Genetic analysis was carried out with the same dose but administered
for a period of 360 days.
Results: CYP enzyme activity increased significantly (p<0.01) in the ND treated group of animals
compared to that in the normal group. However, no noticeable alteration was observed at the molecular
Conclusion: From the present study it could be inferred that, at elevated doses, ND has the potential
to alter hepatic CYP enzyme activity without any modification in the CYP gene. This could be due to
a possible adaptive response of the living system to such drugs.