Description: Design methods of nanoparticle formulations are divided into break-down
methods and build-up methods. The former is further divided into dry and wet processes. For drug
nanoparticle preparations, the wet process is generally employed, and organic solvents are used in
Method: In this study, we investigate the preparation of nifedipine (IB) and griseofulvin (GF)
nanoparticles without using organic solvent. Both IB and GF nanoparticles, with a mean particle size
of approximately 50 nm, were prepared without organic solvent by employing a combination of roll
milling and high-pressure homogenization.
Result: The X-ray diffraction peak of the IB and GF samples prepared by roll milling was present at
a position (2θ) identical to that of IB and GF crystals, indicating that no peak shift was induced by
interaction with phospholipids.
Conclusion: These findings demonstrate that most IB and GF nanoparticles exist as crystals in phospholipids.