The Regulation of Recombinant Protein S Secretion by Extracellular Factors

Author(s): Vijaya S. Pilli, Rinku Majumder*.

Journal Name: Current Chemical Biology

Volume 12 , Issue 1 , 2018

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Abstract:

Background: Protein S (PS) is a natural anticoagulant that has a vital function in regulating hemostasis and inflammation. Recently, the physiological and functional aspects of PS have gained considerable attention, whereas the biophysical properties of PS are far less well defined.

Objective: In this study, our goal was to improve the yield of recombinant PS with minimal contamination by endogenous, host cell PS.

Method: PS-stable cell lines were exposed to external factors and analyzed by qPCR and immunoblotting for endogenous and exogenous PS levels.

Results: Glucose treatment decreased the overall yield of endogenous and exogenous PS, hydrogen peroxide treatment increased the yield of both endogenous and exogenous PS, and calcium chloride treatment increased secretion of exogenous PS without affecting secretion of endogenous PS.

Conclusion: Supplementation of the expression medium with 5 mM calcium chloride improves the yield of recombinant PS.

Keywords: Protein S, transcription, secretion, H2O2, CaCl2, glucose.

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Article Details

VOLUME: 12
ISSUE: 1
Year: 2018
Page: [100 - 103]
Pages: 4
DOI: 10.2174/2212796811666171109121623

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