Antioxidant Activity, Total Flavonoid and Phenolic Contents of Three Different Extracts of Hyrcanian Reishi

Author(s): Somayeh Keypour, Foroogh Mirzania*, Mahdi M. Farimani.

Journal Name: Current Bioactive Compounds

Volume 15 , Issue 1 , 2019

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Abstract:

Background: It has been decades since natural biomaterials, including mushrooms, are examined for antioxidant capacity to put them in the place of the synthetic antioxidants causing cancer. Ganoderma lucidum Karst is an annual fungus reputed for possessing medicinal properties. The fungus has a high potential to be used as a dietary supplement or a source of nutrients and antioxidant agents. It has not been more than a decade since the scientists are working on the different medicinal properties of the endemic Ganoderma lucidum in Iran. This study was conducted, in order to complete a part of this goal and comparing the antioxidant potential of the endemic specimen from Iran with findings from other countries. The aim of this investigation was to test the antioxidant properties, total flavonoid and phenolic contents of various extracts of Ganoderma lucidum in the form of fruit bodies.

Methods: Mushroom samples were extracted with chloroform, methanol and water by maceration method. Ferric Reducing Antioxidant Power, DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'- azino-bis[3-ethylbenzothiazoline-6-sulphonic acid]) were methods used for antioxidant studies in this work. Also, total flavonoid and phenolic contents of these extracts were evaluated.

Results: The methanol extraction illustrated the highest radical scavenging capacity 21.51±0.90 µmoL Trolox/g Ferric reducing assay. The best activities in ABTS and DPPH tests were obtained by chloroform extracts with the 31.36±2.30 µmoL Trolox/g and 6.07±0.08 µg/mL, respectively. Also the chloroform extraction of this fungus displayed the highest total phenolic and flavonoid content 167.75±1.27 GAE/g and 38.00±0.75 mg quercetin equivalents/g, respectively.

Conclusion: The results displayed that Reishi might be proposed as a source of natural antioxidant compounds and can be used as excellent food supplement.

Keywords: Ganoderma lucidum, antioxidant, fruiting body, total flavonoid, phenolic content, plant exracts.

[1]
Adachi, H.; Fujiwara, Y.; Ishii, N. Effects of oxygen on protein carbonyl and aging in Caenorhabditis elegans mutants with long (age-1) and short (mev-1) life spans. J. Gerontol. A Biol. Sci. Med. Sci., 1998, 53(4), B240-B244.
[2]
Agarwal, S.; Sohal, R.S. Relationship between aging and susceptibility to protein oxidative damage. Biochem. Biophys. Res. Commun., 1993, 194(3), 1203-1206.
[3]
Aksenova, M.V.; Aksenov, M.Y.; Carney, J.M.; Butterfield, D.A. Protein oxidation and enzyme activity decline in old brown Norway rats are reduced by dietary restriction. Mech. Ageing Dev., 1998, 100(2), 157-168.
[4]
Chandra, M.; Chandra, N.; Agrawal, R.; Kumar, A.; Ghatak, A.; Pandey, V.C. The free radical system in ischemic heart disease. Int. J. Cardiol., 1994, 43(2), 121-125.
[5]
Wasser, S.P.; Weis, A.L. Therapeutic effects of substances occurring in higher Basidiomycetes mushrooms: A modern perspective. Crit. Rev. Immunol., 1999, 19(1), 65-96.
[6]
Lawal, T.; Wicks, S.; Mahady, G. Ganoderma lucidum (Ling-zhi): The Impact of Chemistry on Biological Activity in Cancer. Curr. Bioact. Compd., 2017, 13, 28-40.
[7]
Ohkawa, H.; Ohishi, N.; Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 1979, 95(2), 351-358.
[8]
Mau, J.L.; Lin, H.C.; Chen, C.C. Antioxidant properties of several medicinal mushrooms. J. Agric. Food Chem., 2002, 50(21), 6072-6077.
[9]
Akiyama, H.; Fujii, K.; Yamasaki, O.; Oono, T.; Iwatsuki, K. Antibacterial action of several tannins against Staphylococcus aureus. J. Antimicrob. Chemother., 2001, 48(4), 487-491.
[10]
Pietta, P.G. Flavonoids as antioxidants. J. Nat. Prod., 2000, 63(7), 1035-1042.
[11]
Kim, M.Y.; Seguin, P.; Ahn, J.K.; Kim, J.J.; Chun, S.C.; Kim, E.H.; Seo, S.H.; Kang, E.Y.; Kim, S.L.; Park, Y.J.; Ro, H.M.; Chung, I.M. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J. Agric. Food Chem., 2008, 56(16), 7265-7270.
[12]
Puttaraju, N.G.; Venkateshaiah, S.U.; Dharmesh, S.M.; Urs, S.M.N.; Somasundaram, R. Antioxidant activity of indigenous edible mushrooms. J. Agric. Food Chem., 2006, 54(26), 9764-9772.
[13]
Koleva, I.I.; van Beek, T.A.; Linssen, J.P.; de Groot, A.; Evstatieva, L.N. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem. Anal., 2002, 13(1), 8-17.
[14]
Benzie, I.F.; Strain, J.J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem., 1996, 239(1), 70-76.
[15]
Keypour, S.; Rafati, H.; Riahi, H.; Mirzajani Demneh, F.; Moradali, M.F. Separation and identification of different ganoderic acids from Ganoderma lucidum of East and West of Asia by hyphenated RP-HPLC and mass spectrometry. Food Chem., 2010, 119(4), 1704-1708.
[16]
Keypour, S.S.; Riahi, H.; Rafati, H.; Moradali, M.F. Investigation of antibacterial effect of Ganoderma lucidum water extract from Iran. J. Med. Plant, 2009, 32, 53-59.
[17]
Keypour, S.; Riahi, H.; Moradali, M.F.; Rafati, H. Investigation of the antibacterial activity of a chloroform extract of Ling Zhi or Reishi medicinal mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst.(Aphyllophoromycetideae), from Iran. Int. J. Med. Mushrooms, 2008, 10(4), 84-93.
[18]
Moradali, M.F.; Hedjaroude, G.A.; Mostafavi, H.; Abbasi, M.; Ghods, S.; Sharifi-Tehrani, A. The genus Ganoderma (Basidiomycota) in Iran. Mycotaxon, 2007, 99, 251-269.
[19]
Xu, X.; Xie, H.; Wang, Y.; Wei, X. A-type proanthocyanidins from lychee seeds and their antioxidant and antiviral activities. J. Agric. Food Chem., 2010, 58(22), 11667-11672.
[20]
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 1999, 26(9-10), 1231-1237.
[21]
Zhou, K.; Su, L.; Yu, L.L. Phytochemicals and antioxidant properties in wheat bran. J. Agric. Food Chem., 2004, 52(20), 6108-6114.
[22]
Zhishen, J.; Mengcheng, T.; Jianming, W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 1999, 64(4), 555-559.
[23]
Andarwulan, N.; Batari, R.; Sandrasari, D.A.; Bolling, B.; Wijaya, H. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem., 2010, 121(4), 1231-1235.
[24]
Ghasemi, K.; Ghasemi, Y.; Ebrahimzadeh, M.A. Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues. Pak. J. Pharm. Sci., 2009, 22(3), 277-281.
[25]
Rafieian-Kopaei, M.; Baradaran, A.; Rafieian, M. Plants antioxidants: From laboratory to clinic. J. Nephropathol., 2013, 2(2), 152-153.
[26]
Karaman, M.; Jovin, E.; Malbaša, R.; Matavuly, M.; Popović, M. Medicinal and edible lignicolous fungi as natural sources of antioxidative and antibacterial agents. Phytother. Res., 2010, 24(10), 1473-1481.
[27]
Wang, J.; Hu, S.; Nie, S.; Yu, Q.; Xie, M. Reviews on mechanisms of in vitro antioxidant activity of polysaccharides; Oxid Med Cell, 2016, p. 13.
[28]
Gąsecka, M.; Mleczek, M.; Siwulski, M.; Niedzielski, P.; Kozak, L. Phenolic and flavonoid content in Hericium Erinaceus, Ganoderma Lucidum, and Agrocybe Aegerita under selenium addition. Acta Aliment., 2016, 45(2), 300-308.
[29]
Vazirian, M.; Dianat, S.; Manayi, A.; Ziari, R.; Mousazadeh, A.; Habibi, E.; Amanzadeh, Y. Anti-inflammatory effect, total polysaccharide, total phenolics content and antioxidant activity of the aqueous extract of three basidiomycetes. Res. J. Pharmacognosy, 2014, 1(1), 15-21.
[30]
Modi, H.A.; Shah, P.; Shukla, M.D.; Lahiri, S.K. Determination of total phenolic content and antioxidant activity of Ganoderma lucidum collected from Dang district of Gujarat, India. Nat. Prod. Ind. J., 2014, 10(3), 75-83.
[31]
Rajasekaran, M.; Kalaimagal, C. In vitro antioxidant activity of ethanolic extract of a medicinal mushroom, Ganoderma lucidum. Int. J. Appl. Pharm. Sci. Res., 2011, 3, 1427-1433.


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Article Details

VOLUME: 15
ISSUE: 1
Year: 2019
Page: [109 - 113]
Pages: 5
DOI: 10.2174/1573407213666171107151007
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