Objective: To explore the expression profile of angiogenic factors associated
with proliferative diabetic retinopathy (PDR).
Methods: Undiluted vitreous humor samples were obtained from 10 diabetic patients
with PDR (10 eyes) and 9 non-diabtic patients (9 eyes). The concentrations of 60
angiogenic factors in the vitreous humor samples were measured by RayBio Angiogenic
Cytokine Antibody Array. Some differentially expressed factors were further confirmed in
vitreous humor by enzyme-linked immunosorbent assay (ELISA).
Results: Compared with the non-diabetic controls, 20 differentially expressed factors
with more than 1.50 fold changes were detected in patients with PDR. The median
concentration of vascular endothelial growth factor (VEGF), interleukin 6 (IL-6),
angiopoietin (ANG)-1, ANG-2, urokinase-type plasminogen activator receptor (uPAR),
Follistatin and matrix metalloproteinases 9 (MMP-9) was significantly increased in
vitreous samples from PDR compared to controls (P < 0.05). However, (MCP)-1,
Angiogenin and Leptin was significantly lower in PDR eyes compared to controls (P <
0.05). In the verification assay using ELISA, ANG-1, ANG-2, IL-6, VEGF, MMP-9,
hepatocyte growth factor (HGF) and placenta growth factor (PIGF) concentration were
increased in patients with PDR compared to controls (all P-values < 0.05).
Conclusion: This is the first report of a comprehensive multiplex analysis to identify
angiogenic factors associated with PDR. These angiogenic factors may contribute to the
pathogenesis of PDR and may be targets for therapeutic strategies of PDR.