Cytotoxic Effects and Production of Cytokines Induced by the Endophytic Paenibacillus polymyxa RNC-D In Vitro

Author(s): Debora M. Neris*, Genoveva L.F. Luna, Joice M. de Almeida Rodolpho, Ana C. Urbaczek, Ricardo de Oliveira Correia, Karina A. Feitosa, Nadja F.G. Serrano, Cristina P. de Sousa, Fernanda de Freitas Anibal.

Journal Name: Current Pharmaceutical Biotechnology

Volume 18 , Issue 9 , 2017

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Abstract:

Background: Prominent among all the organisms that have a potential value for the production of new medicines, are endophytes, fungi and bacteria that live inside plants without harming them. In this study, a total lyophilized extract (TLE) of Paenibacillus polymyxa RNC-D was used. The P. polymyxa lineages are known for their capacity to segregate a large number of extracellular enzymes and bioactive substances.

Methods: The TLE of Paenibacillus polymyxa RNC-D was tested in cell viability assays for cytotoxicity and cytokine production in BALB/3T3 and J774A.1 cell lineages.

Results: A 50% mortality rate of fibroblasts (BALB/3T3) was observed in the 1.171±0.161 mg/mL and 0.956±0.112 mg/mL doses after 48 and 72 hours, respectively, as well as a 50% mortality rate of macrophage cells (J774A.1) in the 0.994±0.170 mg/mL and 0.945±0.280 mg/mL doses after 48 and 72 hours, respectively. The ≈1 mg/mL concentration significantly affected the kinetic of growth in all the measured periods. The extract induced apoptosis and necrosis 24 hours after the ≈1 mg/mL concentration in both tested lineages. The treatment with the ≈1 mg/mL concentration led to the production of TNF-α and IFN-γ cytokines in 24 hours. IL-12 and IL-10 began to be detected as a result of the treatment with 0.1 mg/mL. However, with the 0.5 mg/mL dose in 24 hours, a significant reduction in IL-10 was observed.

Conclusion: Our data suggest that the TLE of P. polymyxa RNC-D modulated the production of cytokines with different patterns of immune response in a dose-dependent way.

Keywords: Cell death, cytokines, cytotoxicity, endophytic microorganism, fluorescence, immune response, Paenibacillus polymyxa.

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Article Details

VOLUME: 18
ISSUE: 9
Year: 2017
Page: [758 - 768]
Pages: 11
DOI: 10.2174/1389201018666171026155522
Price: $58

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