Identification of Secretome of MRC-5 Fibroblast Cell Line Using Two-dimensional Electrophoresis Coupled to Mass Spectrometry

Author(s): Zahra Mojtahedi, Kazuyuki Nakamura, Yasuhiro Kuramitsu, Abbas Ghaderi, Jamal Sarvari*.

Journal Name: Current Proteomics

Volume 15 , Issue 1 , 2018

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Abstract:

Objective: Secreted proteins from fibroblasts critically contribute to tumor microenvironment. Human embryonic lung fibroblast MRC-5cell line is a widely used model in cancer formation and progression studies. However, data regarding its secretome are poor.

Method: In the present study, we used immobilized pH gradient-based two dimensional gel electrophoresis (2DE) and mass spectrometry to characterize the secretome of MRC-5 cell line.

Results: In total, 21 protein spots related to 17 proteins were identified. Some of them were insulin-like growth factor-binding protein 5, epididymal secretory protein E1 (Niemann-Pick disease type C2 protein), cathepsin L, glutathione S-transferase P, ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5), UPF0587 protein C1orf123, transgelin, cofilin-1, cofilin-2, and guanine nucleotide-binding protein subunit beta-2-like 1.

Conclusion: Using 2DE and mass spectrometry, we identified several proteins in the conditioned media of MRC-5 cell line. The identified proteins were known to be involved in the key cellular processes. Our findings shed more light on the proteome mapping of secreted proteins from a fibroblast cell line.

Keywords: Biomarkers, cancer, mass spectrometry, MRC-5, secretome, two-dimensional electrophoresis.

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Article Details

VOLUME: 15
ISSUE: 1
Year: 2018
Page: [77 - 82]
Pages: 6
DOI: 10.2174/1570164614666171017154608
Price: $25

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