Review on the Application of Mixed-mode Chromatography for Separation of Structure Isoforms

Author(s): Tsutomu Arakawa* .

Journal Name: Current Protein & Peptide Science

Volume 20 , Issue 1 , 2019

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Graphical Abstract:


Proteins often generate structure isoforms naturally or artificially due to, for example, different glycosylation, disulfide scrambling, partial structure rearrangement, oligomer formation or chemical modification. The isoform formations are normally accompanied by alterations in charged state or hydrophobicity. Thus, isoforms can be fractionated by reverse-phase, hydrophobic interaction or ion exchange chromatography. We have applied mixed-mode chromatography for fractionation of isoforms for several model proteins and observed that cation exchange Capto MMC and anion exchange Capto adhere columns are effective in separating conformational isoforms and self-associated oligomers.

Keywords: Mixed-mode, isoform, glycoprotein, arginine, Capto MMC, Capto adhere.

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Article Details

Year: 2019
Page: [56 - 60]
Pages: 5
DOI: 10.2174/1389203718666171009111033
Price: $58

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