Effect of Processing and Frozen Storage on the Phenolic Profile, Bioative Compounds, Antioxidant Capacity, and Enzymatic Activity of Mangaba Pulp

Author(s): Gilma A.S. Gonçalves*, Nathane S. Resende, Elisângela E.N. Carvalho, Jaime V. de Resende, Eduardo V. de B Vilas Boas.

Journal Name: Current Nutrition & Food Science

Volume 15 , Issue 1 , 2019

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Abstract:

Backgound: The mangaba, an exotic fruit of the Brazilian Cerrado, has high nutritional and bioactive value, but processing can induce changes to these characteristics.

Objective: Evaluate the stability of the bioactive compounds and the antioxidant and enzymatic capacity of mangaba pulp subjected to pasteurization, freezing methods, and prolonged storage.

Method: The pulps were submitted to two levels of pasteurization (unpasteurized and pasteurized), two freezing methods (static air and forced air), and five frozen storage times (0, 3, 6, 9, and 12 months). The vitamin C, carotenoids, total phenolics, profile of phenolic compounds, antioxidant capacity, and enzymes polyphenoloxidase and peroxidase were analyzed in the fruit and pulps.

Results: The fruit showed a high vitamin C and total phenolics content and a high antioxidant capacity, including chlorogenic acid and routine predominant phenolics. The forced air freezing method is more efficient in vitamin C retention, and pasteurization favors higher retention of total phenolics and antioxidant capacity, as well as lower enzymatic activity of polyphenoloxidase and peroxidase. Frozen storage for up to six months retains most of the individual phenolics, with (+) - catechin and transcinnamic acid being the most stable.

Conclusion: Mangaba pulp is a good source of bioactive compounds and has good antioxidant capacity, even after one year of frozen storage.

Keywords: Enzymatic browning, exotic fruit, freezing, Hancornia speciosa Gomes, HPLC, pasteurization.

[1]
Dong J, Zhang X, Zhang L, et al. Quercetin reduces obesity-associated ATM infiltration and inflammation in mice: A mechanism including AMPK alpha 1/SIRT1. J Lipid Res 2014; 55: 363-74.
[2]
Mursu J, Virtanen JK, Tuomainen TP, et al. Intake of fruit, berries, and vegetables and risk of type 2 diabetes in Finnish men: The kuopioischaemic heart disease risk factor study. Am J Clin Nutr 2014; 9: 328-33.
[3]
Zhang X, Shao X. Characterisation of polyphenol oxidase and peroxidase and the role in browning of loquat fruit. Czech J Food Sci 2015; 33: 109-17.
[4]
Bailão EFLC, Devilla IA, da Conceição EC, Borges LL. Bioactive compounds found in Brazilian Cerrado fruits. Int J Mol Sci 2015; 16: 23760-83.
[5]
Ganga RM, Ferreira GA, Chaves LJ, et al. Caracterização de frutos e árvores de populações naturais de Hancornia speciosa. Gomes do cerrado. Rev Bras Frutic Jaboticabal 2010; 32(1): 101-13.
[6]
Cardoso MC, Reis BL, Oliveira DS, Pinheiro-Sant’ana HM. Mangaba (Hancornia speciosa Gomes) from the Brazilian Cerrado: Nutritional value, carotenoids and antioxidants vitamins. Fruits 2014; 69: 89-99.
[7]
de Lima JP, Fante CA, Pires CRF, et al. The antioxidative potential and volatile constituents of mangaba fruit over the storage period. Sci Hortic 2015a; 194: 1-6.
[8]
de Lima JP, Azevedo L, de Souza NJ, et al. First evaluation of the antimutagenic effect of mangaba fruit in vivo and its phenolic profile identification. Food Res Int 2015b; 75: 216-24.
[9]
Pereira AC, Pereira ABD, Moreira CCL, et al. Hancorniaspeciosa Gomes (Apocynaceae) as a potentialanti-diabeticdrug. J Ethnopharmacol 2015; 161: 30-5.
[10]
Vieira Neto RD, Cintra FLD, Silva AL, et al. Sistema de produção de mangaba para os tabuleiros costeiros e baixada litorânea. Embrapa Tabuleiros Costeiros: Aracaju 2002.
[11]
Souza LG, Moura AS, Carnelossi MAG, Castro AA. Avaliação dos níveis de Aceitação e de intenção de compra da polpa de laranja pera congelada e criocongelada. Cientia Plena 2011; 7(3): 1-8.
[12]
García-Reyes RH, Narvaez-Cuenca CE. The effect of pasteurization on the quality of frozen arazá (Eugenia StipitataMcVaugh) pulp. J Food Qual 2010; 33: 632-45.
[13]
Oszmiański J, Wolniak M, Wojdyło A, Wawer I. Influence of apple purée preparation and storage on polyphenol contents and antioxidant activity. Food Chem 2008; 107: 1473-84.
[14]
Sousa MB, Canet W, Alvarez MD, Fernández C. Effect of processing on the texture and sensory attributes of raspberry (cv Heritage) and blackberry (cv Thornfree). J Food Eng 2007; 78: 9-21.
[15]
Zaro MJ, Keunchkarian S, Chaves AR, et al. Changes in bioactive compounds and response to postharvest storage conditions in purple eggplants as affected by fruit developmental stage. Postharvest Biol Technol 2014; 96: 110-7.
[16]
Martim SR, Neto JC. de A Oliveira IM. Physicochemical characteristics and enzymatic activity of peroxidase and polyphenoloxidase in four genotypes of cupuaçu (Theobroma grandiflorum Willd ex-Spreng Schum) submitted to freezing. Semina. Ciênc Agrár Londrina 2013; 34: 2265-76.
[17]
Oliveira LS, Moura CFH, Brito ES, et al. Quality changes and anthocyanin and vitamin C decay rates of frozen acerola purée during long-term storage. J Food Process Preserv Westport 2013; 37: 25-33.
[18]
Zhang Q, Chen W, Zhao J, Xi W. Functional constituents and antioxidant activities of eight Chinese native goji genotypes. Food Chem 2016; 200: 230-6.
[19]
Strohecker R, Henning HM. Analisis de vitaminas: Métodos comprobados. Paz Montalvo: Madrid 1967.
[20]
Rodriguez-Amaya DB. A guide to carotenoids analysis in foods. Ilsi: Washington 1999.
[21]
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 1995; 28(1): 25-30.
[22]
Rufino MSM, Alves RE, Brito ES, et al. Metodologia científica: Determinação da atividade antioxidante total em frutas pela captura do radical livre DPPH. Comunicado Técnico 2007; 127: 1-4.
[23]
Waterhouse AL. Polyphenolics: Determination of total phenolics. In: Wrolstad RE, Ed.Current protocols in food analytical chemistry. J Wiley: New York 2002; pp. 1111-8.
[24]
Ramaya SD, Bujang JS, Zakaria MH, et al. Sugar, ascorbic acid, total phenolic content and total antioxidant activity in passion fruit (Passiflora) cultivars. J Sci Food Agric 2012; 93(5): 1198-205.
[25]
Ozgen M, Reese RN, Tulio Jr A.Z., et al. Modified 2,2-azinobis-3-ethylbonzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) methods. J Agric Food Chem 2006; 54: 1151-7.
[26]
Matsuno H, Uritani I. Physiological behavior of peroxidase isozymes in sweet potato root tissue injured by cutting or with black rot. Plant Cell Physiol Tóquio 1972; 13: 1091-101.
[27]
Ferreira DF. SISVAR: Sistema de análise de variância. Versão 5.3. Lavras-MG: UFLA 2010.
[28]
Rufino MSM, Alves RE, Brito ES, et al. Bioactive compounds and antioxidant capacities of 18 non-traditional tropical fruits from Brazil. Food Chem 2010; 121: 996-1002.
[29]
Almeida MMB, Sousa PHM, Arriaga ÂMC, et al. Bioactive compounds and antioxidant activity of fresh exotic fruits from north eastern Brazil. Food Res Int 2011; 44: 2155-9.
[30]
Ali SS, Kasoju N, Luthra A, et al. Indian medicinal herbs as sources of antioxidants. Food Res Int 2008; 41(1): 1-15.
[31]
Mantovani C, Clemente E. Peroxidase and polyphenoloxidase activity in tomato in natura and tomato purée. Acta Sci Technol 2010; 32: 91-7.
[32]
Nasser FA de CM. Prado HFA, Boliani AC, Nasser MD. Eficiência de soluções extratoras para análise da peroxidase e polifenoloxidase em mangaba. Tecnol Ciên Agropec, João Pessoa 2014; 8(2): 69-72.
[33]
Vitorino LC, de Moura LC, Oliveira C, et al. Influência da temperatura e do tempo de armazenamento sobre a atividade de enzimas oxidativas em Hancornia speciosa Gomes. Agrotropica 2014; 26(3): 149-56.
[34]
Faniadis D, Drogoudi PD, Vasilakakis M. Effects of cultivar, orchard elevation, and storage on fruit quality characters of sweet cherry (Prunusavium L.). Sci Hortic 2010; 125(3): 301-4.
[35]
Gonçalves GAS, Resende NS, Gonçalves CS, et al. Temporal dominance of sensations for characterization of strawberry pulp subjected to pasteurization and different freezing methods. LWT -. Food Sci Technol (Lond) 2017; 77: 413-21.
[36]
Reno MJ, Prado MET, Resende JV. Microstructural changes of frozen strawberries submitted to pre-treatments with additives and vacuum impregnation. Cienc Tecnol Aliment 2011; 31: 247-56.
[37]
Bezerra TS, Fernandes TN, Resende JV. Effects of added sucrose and pectin on the rheological behavior and freezing kinetics of passion fruit pulp studied by response surface methodology. J Food Sci Technol 2015; 52(6): 3350-7.
[38]
Oliveira LS, Rufino SMR, Moura CFH, et al. The influence of processing and long-term storage on the antioxidant metabolism of acerola (Malpighiae marginata) purée. Braz J Plant Physiol Londrina 2011; 23(2): 151-60.
[39]
Institute of Medicine, Food and Nutrition Board. Dietary reference intakes. National Academic Press: Washington, DC 2011.
[40]
Poiana M, Moigradean D, Raba D, et al. The effect of long-term frozen storage on the nutraceutical compounds, antioxidant properties and color indices of different kinds of berries. J Food Agric Environ 2010; 8: 54-8.
[41]
Damiani C, Lage ME, Silva FA, et al. Changes in the physicochemical and microbiological properties of frozen araça pulp during storage. CiêncTecnol Aliment 2013; 33: 19-27.
[42]
Jacques AC, Pertuzatti PB, Barcia MT, et al. Estabilidade de compostos bioativos em polpa congelada de amora-preta (Rubusfruticosus) cv. Tupy. Quim Nova 2010; 33: 1720-5.
[43]
Leong SY, Oey I. Effects of processing on anthocyanins, carotenoids and vitamin C in summer fruits and vegetables. Food Chem 2012; 133: 1577-87.
[44]
Zhang Q, Chen W, Zhao J, Xi W. Functional constituents and antioxidant activities of eight Chinese native goji genotypes. Food Chem 2016; 200: 230-6.
[45]
Gomes EDB, Ramalho SA, Gualberto NC. A rapid method for determination of some phenolic acids in Brazilian tropical fruits of mangaba (Hancornia speciosa Gomes) and umbu (Spondias tuberosa Arruda Camara) by UPLC. J Anal Sci Meth Instrum 2013; 3: 1-10.
[46]
Ferreira HC, Serra CP, Endringer DC, et al. Endothelium-dependent vasodilation induced by Hancornia speciosa in rat superior mesenteric artery. Phytomedicine 2007; 14: 473-8.
[47]
Sun Q, Heilmann J, Konig B. Natural phenolic metabolites with anti-angiogenic properties: A review from the chemical point of view. Beilstein J Org Chem 2015; 11: 249-64.
[48]
Friedman M, Jürgens HS. Effect of pH on the Stability of Plant Phenolic Compounds. J Agric Food Chem 2000; 48(6): 2101-10.
[49]
Chang SK, Alasalvar C, Bolling BW, Shahidi S. Nuts and their co-products: The impact of processing (roasting) on phenolics, bioavailability, and health benefits - A comprehensive review. J Funct Foods 2016; 26: 88-122.
[50]
Oliveira A, Almeida DPF, Pintado M. Changes in Phenolic Compounds During Storage of Pasteurized Strawberry. Food Bioprocess Technol 2014; 7: 1840-6.
[51]
Chandrasekara N, Shahidi F. Effect of roasting on phenolic content and antioxidant activities of whole cashew nuts, kernels, and testa. J Agric Food Chem 2011; 59(9): 5006-14.
[52]
Guo Q, Zhao B, Shen S, Hou J, Hu J, Xin W. ESR study on the structure antioxidant activity relationship of tea catechins and their epimers. Biochim Biophys Acta 1999; 1427: 13-23.
[53]
Fuleki T, Ricardo-da-Silva JM. Effects of cultivar and processing method on the contents of catechins and procyanidins in grape juice. J Agric Food Chem 2003; 51(3): 640-6.
[54]
Vallverdú-Queralt A, Medina-Remón A, Casals-Ribes I, Lamuela-Raventos RM. Is there any difference between the phenolic content of organic and conventional tomato juices? J Agric Food Chem 2012; 60(38): 9667-72.
[55]
Yu J, Ahmedna M, Goktepe I, et al. Peanut skin procyanidins: Composition and antioxidant activities as affected by processing. J Food Compos Anal 2006; 19: 364-71.
[56]
Rohn S, Buchner N, Driemel G, et al. Thermal degradation of onion quercetin glucosides under roasting conditions. J Agric Food Chem 2007; 55: 1568-73.
[57]
Buchner N, Krumbein A, Rohn S, et al. Effect of thermal processing on the flavonols rutin and quercetin. Rapid Commun Mass Spectrom 2006; 20: 3229-35.
[58]
Friedman M. Dietary impact of food processing. Annu Rev Nutr 1992; 12: 119-37.
[59]
Vallverdú-Queralt A, Medina-Remón A, Casals-Ribes I, Lamuela-Raventos RM. Is there any difference between the phenolic content of organic and conventional tomato juices? J Agric Food Chem 2012; 60(38): 9667-72.
[60]
Manach C, Scalbert A, Morand C, et al. Polyphenols: Food sources and bioavailability. Am J Clin Nutr 2004; 79: 727-47.
[61]
Hvattum E, Ekeberg D. Study of the collision-induced radical cleavage of flavonoid glycosides using negative electrospray ionization tandem quadrupole mass spectrometry. J Mass Spectrom Chichester 2003; 38(1): 43-9.
[62]
Álvarez-Fernández M, Hornedo-Ortega AR, Cerezo AB, et al. Effects of the strawberry (Fragaria ananassa) purée elaboration process on non-anthocyanin phenolic composition and antioxidant activity. Food Chem 2014; 164: 104-12.
[63]
Parker TL, Esgro ST, Miller SA, et al. Development of an optimised papaya pulp nectar using a combination of irradiation and mild heat. Food Chem 2010; 118: 861-9.
[64]
Zaro MJ, Keunchkarian S, Chaves AR, et al. Changes in bioactive compounds and response to postharvest storage conditions in purple eggplants as affected by fruit developmental stage. Postharvest Biol Technol 2014; 96: 110-7.
[65]
Freire JM, Abreu CMP, Rocha DA, et al. Quantificação de compostos fenólicos e ácido ascórbico em frutos e polpas congeladas de acerola, caju, goiaba e morango. Cienc Rural, Santa Maria 2013; 43(12): 2291-5.
[66]
Menolli LN, Finger FL, Puiatti M, et al. Atuação das enzimas oxidativas no escurecimento causado pela injúria por frio em raízes de batata-baroa. Acta Sci Agron 2008; 30: 57-63.
[67]
Zhang X, Shao X. Characterisation of polyphenol oxidase and peroxidase and the role in browning of loquat fruit. Czech J Food Sci 2015; 33: 109-17.
[68]
Vanini LS, Kwiatkowski A, Clemente E. Polyphenoloxidase and peroxidase in avocado pulp (Persea americana Mill.). Ciênc Tecnol Aliment Campinas 2010; 30(2): 525-31.


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Article Details

VOLUME: 15
ISSUE: 1
Year: 2019
Page: [48 - 60]
Pages: 13
DOI: 10.2174/1573401313666171004144858
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