Herbal Mixture Adsorbed to Polyethylene Glycol Microspheres Induces Apoptotic Effects on Breast Cancer Cells

Author(s): Aliny Aparecida Lopes Ribeiro , Fabiana Helen da Silva , Aron Carlos de Melo Cotrim , Alessandra Lima Deluque , Patricia Gelli Feres de Marchi , Danny Laura Gomes Fagundes , Claudia de Sousa Pereira , Eduardo Luzia Franca , Adenilda Cristina Honorio-Franca* .

Journal Name: Current Drug Delivery

Volume 15 , Issue 2 , 2018

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Abstract:

Background: The combination of medicinal plants and polymeric matrices raised the possibility of obtaining new drugs to treat a number of diseases, including cancer. Breast cancer is one of the most frequent diseases among women, but the effective treatments is still a challenge.

Methods: Thus, this study investigated the effect of herbal mixture adsorbed to PEG microspheres on MCF-7 human breast cancer cells and these cells in co-culture of blood MN cells. The MCF-7 cells and the blood mononuclear [MN] cells from volunteer donors. MN cells, MCF-7 cells and co-culture [MN and MCF-7 cells] were pre-incubated for 24 h with or without herbal mixture [HM], polyethylene glycol [PEG] microspheres or herbal mixture adsorbed in PEG microspheres [PEG-HM]. Viability, intracellular calcium and apoptosis were determined by flow cytometry. The herbal mixture adsorbed in PEG microspheres reduces the viability of MCF-7 cells.

Results: The lowest viability índex was observed in co-culture incubated with herbal mixture adsorbed to PEG microspheres. MN cells, MCF-7 cells and co-culture showed higher intracellular Ca2+ release when incubated with herbal mixture adsorbed to PEG microspheres. The apoptosis index was higher in MCF-7 cells that were treated with herbal mixture. The highest apoptosis index was observed in coculture of these cells incubated with herbal mixture adsorbed to PEG microspheres.

Conclusion: These data suggest that herbal mixture adsorbed by PEG microspheres has apoptotic effects on human MCF-7 breast cancer cells and is therefore a possible therapeutic alternative.

Keywords: PEG Microspheres, herbal mixture, MCF-7, MN cells, apoptosis, cancer.

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Article Details

VOLUME: 15
ISSUE: 2
Year: 2018
Page: [227 - 234]
Pages: 8
DOI: 10.2174/1567201814666171002141430
Price: $58

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