Background: Green electrochemical sensor (an in-situ plated bismuth film glassy carbon
electrode) was used for the first time in combination with adsorptive striping voltammetry to elaborate
the novel, simple, cheap and sensitive alternative analytical procedure for determination of rutin.
Methods and Results: In this paper, the electrochemical procedure for the determination of rutin using a
square-wave adsorptive stripping voltammetry and a bismuth film modified glassy carbon electrode was
developed. Rutin provided a well-defined, single oxidation peak at +0.45 V (vs. Ag/AgCl) in the solution
containing 0.025 mol L-1 of acetate buffer (pH = 4.0 ± 0.1), 5.0 × 10-7 mol L-1 Bi(NO3)3. Under
optimum conditions, the obtained results showed a linear calibration graph of the peak current (Ip) versus
rutin concertation in the range of 1.0 × 10-10 to 1.0 × 10-8 mol L-1 with a correlation coefficient of
0.9969. The determined detection limit was 2.2 × 10-11 mol L-1 for the accumulation time of 60 s. The
sensor prepared in situ was successfully applied to the determination of rutin content in the tablets without
Conclusion: In this paper, significant progress has been made in the development of suitable electrochemical
procedure for rutin determination in comparison to our previous paper as well as other works
described in the literature, due to the application of bismuth film electrode.