Background: Phytases are enzymes capable of degrading phytic acid and are used in animal feed
supplementation in order to improve digestibility through the release of minerals such as phosphorus. Recent
inventions show interest in production and optimization of recombinant phytases with biochemical and
physicochemical characteristics promising for animal feed industry.
Methods: This review article is focused on relevant patents of promising phytases, together with the commonly
used expression systems for their production and tools currently employed to generate new phytases.
We revised all patents related to recombinant phytases and their application in the animal feed industry. The
following patents databases were consulted: European Patent Office (Espacenet), the United States Patent
and Trademark Office (USPTO), the United States Latin America Patents (LATIPAT), Patent scope -Search
International and National Patent Collections (WIPO) and Google Patents.
Results: In this review, information was collected from recent publications, including 38 patents related production
systems for different recombinant phytases and their application in the animal feed industry.
Conclusion: We showed that important recombinant phytases were successfully produced in different expression
systems. In addition, this work highlights certain biotechnological tools such as mutagenesis for
generation of novel enzymes with biochemical properties of use in the animal feed industry.