From synthesis to decay, mRNA associates with RNA-binding proteins (RBPs) establishing
dynamic ribonucleoprotein particles (RNPs). Understanding the composition and function of
RNPs is fundamental to understanding how eukaryotic mRNAs are controlled. This is especially
relevant for trypanosomes and related kinetoplastid parasites, which mostly rely on posttranscriptional
mechanisms to control gene expression. Crucial for trypanosome differentiation, development,
or even response to heat shock, RBPs are known to be essential modulators of diverse
molecular processes. The recent application of large-scale quantitative methods, such as Next-
Generation Sequencing (NGS) and quantitative mass spectrometry, has revealed new exciting features
about the parasite RNA-related metabolism. Novel proteins carrying RNA-binding activity,
including many proteins without RNA-related ontology were discovered setting a necessary
groundwork to get in insights into RNA biology.
Conclusion: This review aims to give the reader an understanding of current trypanosome RNP research,
highlighting the progress made using high-throughput approaches.