Quantification of Sulfotransferases 1A1 and 1A3/4 in Tissue Fractions and Cell Lines by Multiple Reaction Monitoring Mass Spectrometry

Author(s): Sho Yoshitake, Melissa McKay-Daily, Masaki Tanaka, Zeqi Huang*.

Journal Name: Drug Metabolism Letters

Volume 11 , Issue 1 , 2017

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Abstract:

Background: Within the sulfotransferase (SULT) superfamily of metabolic enzymes, SULT1A1 and 1A3/4 isoforms are of particular interest, due to their abilities to catalyze the sulfation of phenolic endobiotics and xenobiotics. Although the difference in their substrate specificity is well documented, an isoform-specific quantification method is still not available.

Objective: To detect and quantify SULT1A1 and 1A3/4 in S9 fractions and cell lines using targeted mass spectrometry-based proteomics.

Method: Samples were tryptically digested, and signature peptides were quantified using liquid chromatography- multiple reaction monitoring mass spectrometry (LC-MRM/MS). Stable isotopelabeled (SIL) peptides were used as internal and calibration standards. SULT1A1 and SULT1A3/4 were quantified in various S9 fractions and cell line samples.

Results: Intraday and interday variabilities were low for relative quantification in S9 and cell line matrices (<8%). Expression profiles were validated using Western blot analysis of S9 fractions and lentiviral transduced SULT1A-overexpressing cell lines.

Conclusion: A reproducible method for simultaneous quantification of SULT1A1 and SULT1A3/4 in S9 fractions and cell line samples was established and validated.

Keywords: LC-MRM/MS, SULT, quantitative proteomics, stable isotope labeled peptides, absolute quantification, S9 fractions, cell lines.

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Article Details

VOLUME: 11
ISSUE: 1
Year: 2017
Page: [35 - 47]
Pages: 13
DOI: 10.2174/1872312811666170731170153
Price: $58

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