Background: Over the past five years, the cold atmospheric plasma-activated solutions (PAS) have
shown their promissing application in cancer treatment. Similar as the common direct cold plasma treatment,
PAS shows a selective anti-cancer capacity in vitro and in vivo. However, different from the direct cold atmospheric
plasma (CAP) treatment, PAS can be stored for a long time and can be used without dependence on a
CAP device. The research on PAS is gradually becoming a hot topic in plasma medicine.
Objectives: In this review, we gave a concise but comprehensive summary on key topics about PAS including
the development, current status, as well as the main conclusions about the anti-cancer mechanism achieved in
past years. The approaches to make strong and stable PAS are also summarized.