Background: Human infectious diseases are caused by various pathogens
including bacteria, fungi, viruses, parasites, and protozoans. These infectious agents
are controlled by using synthetic drugs as well as natural sources.
Objective: The aim of current study was to evaluate the antibacterial effect of Rumex
hastatus against clinical bacterial pathogens.
Methods: In current research antibacterial effect of Rumex hastatus was analyzed
against seven clinical pathogenic bacteria such as Escherichia coli, Serratia marcescens,
Streptococcus pyogenes, Staphylococcus epidermidis, Staphylococcus aureus,
Klebsiella pneumoniae and Pseudomonas aeruginosa through agar well diffusion
method. The boiled extract was used for the phytochemical screening, antioxidant
potential, thin layer chromatography, bio-autography, and spot screening. Genomic
DNA was extracted to find the DNA protection effect of R. hastatus.
Results: Antibacterial results showed that diethyl ether extract has the maximum inhibition
of S. pyogenes (9.66 ± 0.57 mm). Acetone and diethyl ether extracts showed
moderate inhibition of K. pneumoniae (6.33 ± 1.52 mm and 5.66 ±1.15 mm) and S.
aureus (6.33 ± 1.52 mm and 5.66 ± 0.57 mm). Similarly, chloroform extract indicated
moderate inhibition of S. pyogenes (5.66 ± 1.15 mm). Ethanol extract had low or
even no effect on the growth of bacteria. Genomic DNA extraction also encouraged
the antibacterial effect of R. hastatus. Various phytochemical constituents such as
ketoses, oligosaccharides, amino acids, amines, sugars, flavonoids, and antioxidant
constituents were detected. TLC-Bioautography and spot screening results revealed
the potential use of R. hustatus as an antibacterial agent.
Conclusion: It was concluded that most of the tested fractions appeared as an important
source for the discovery of new antimicrobial drugs.