Background: Production of Beta-Lactamase enzymes, especially extended-
spectrum Beta -Lactamases (ESBL), is a primary mechanism of resistance in
these bacteria.The purpose of this study was detection of blaTEM, blaSHV, blaCTXM,
blaCTX-M-15, blaPERand blaVEBin K. pneumoniae, isolated from clinical specimens by
the PCR method and antibiotic susceptibility patterns in these strains isolated.
Materials and Methods: During a period from October 2015 to July 2016, 52 K. pneumoniae
isolates were collected from general hospitals in the city of Sanandaj, Iran. After
identifying the strains by biochemical testing, the disc diffusion method was used for determining
antimicrobial susceptibility and screening the ESBL-producing isolates. Detection
of blaTEM, blaSHV, blaCTX-M, blaCTX-M-15, blaPER and blaVEBESBL-producing K. pneumoniae
was carried out by PCR.
Results: Out of 52-collected K. pneumoniae, highest and lowest rates of resistance related
to co-trimoxazole with 67.3 % and amikacin with 30.7 %. 55.7% identified as MDR and
69.23% were ESBL-producing K. pneumoniae.blaSHV was the most prevalent gene in
ESBL-producing K. pneumoniae. blaTEM,blaCTX-M,blaCTX-M-15 producing K. pneumoniae
strains showed higher rates of drug resistant compared with negative strains (P < 0.05).
Conclusion: Results of this study showed that the prevalence rate of ESBL-producing K.
pneumoniae isolates is increasing in MDR strains, which raises concerns regarding the
treatment of K. pneumoniae. Therefore, molecular research in the field of antimicrobial
resistance of bacteria is essential to prevent the spread of resistant strains.