Background: Cancer is one of the major health and socio-economic problems of the world
even though extensive progress has been made in terms of early diagnosis and cure. The research for
new anticancer drug development with more effective treatment is a field of utmost importance in current
Method: Molecular Hybridization (MH) strategy has been adapted for the rational design of new
ligands, where pharmacophoric sub-unities in the molecular structure of two bioactives will lead to the
design of new hybrid architectures. All the synthesized compounds (3a-z) were evaluated in HeLa and
MCF-7 cell lines for their anticancer activity and compared against H9C2 normal cells.
Results: As expected, among the tested compounds, 3a, 3d, 3g, 3j, 3m and 3s exhibited highest activity
with GI50 value of 1.72 µm, 2.10 µm, 4.26 µm, 2.43 µm, 5.11 µm and 4.34µm in HeLa cells and 1.86
µm, 2.50 µm, 5.20 µm, 4.40 µm, 6.14 µm and 5.30 µm in MCF-7 cells respectively. We also found that
the compounds were non-toxic to H9C2 cells up to 20 µM.
Conclusion: Preliminary structure-activity relationship studies revealed that compounds with para-Cl
substituted phenyl and 5-Cl substituted indole on diazepine ring proved to be more potent.