Background: Spermatogonial stem cells (SSCs) are unique in mammals because they can
transmit genetic information from generation to generation and it is of significant importance. In testes,
Sertoli cells, peritubular myoid cells, Leydig cells and other interstitial cells contribute to the
spermatogonial stem cell “niche”. So, creation of niche in an in vitro condition that mimics the in vivo
environment is essential to maintain functional characteristic of SSCs.
Objective: In this review, we describe the impact of nanofiber scaffolds on the culture of SSCs derived
Results: Nanofiber Matrices mimic the architecture and size scale of the natural extracellular matrix
(ECM). The scaffold provides more three-dimensional (3D), biomimicking and topographical signals
to the cells and results in a more physiologically relevant cellular phenotype. Several investigators use
different nanofiber scaffold-like carbon nanotubes (CNTs) scaffold, poly-L-lactic acid (PLLA) nanofiber
scaffold, 3D soft agar culture system, human serum albumin (HSA)/tri calcium phosphate
nanoparticles (TCPNPs) and electrospun polyamide nanofiber for proliferation and maintenance of
self-renewal activity of the SSCs.
Conclusion: Application of nanofiber scaffolds for in vitro culture of the SSCs may produce spermatogonial
stem cells that can be used in regenerative medicine, tissue engineering, assisted reproductive
technology and in the treatment of infertility in pre-pubertal cancer patients.