Background: Papaya (Carica papaya) is well known for its medicinal properties as well
as its use in traditional system of medicine. The recent rise in the knowledge of medicinal properties
of papaya has significantly contributed to the modern pharmaceutical industries.
Objectives: In this study, methanolic extracts of papaya leaves, un-ripened peels and seeds were
studied for their phytochemical composition, antioxidant, anti-inflammatory, antidiabetic and anticancer
Methods: Bioactive compounds were identified using GC-MS analysis. The antioxidant effects of
extracts were estimated by radical scavenging assay. In vitro anti-inflammatory activity was estimated
by human red blood cell membrane stabilisation method. Antidiabetic activity of extracts was
determined by α-amylase inhibition method. The viability of cells was assessed by MTT assay using
MCF-7 and vero cell lines.
Results: The free radical scavenging activity of methanolic extracts of leaf was found to be 67.78%,
un-ripened peel 77.47%, and 90.12% at 1.0 mg/mL concentration. The percentage protection was
calculated to be 75.87%, 91.25% and 88.25% and percentage hemolysis was calculated to be
24.12%, 8.75% and 11.75% of leaf, un-ripened peel and seed extract respectively. α-amylase inhibition
(antidiabetic activity) of 86.93%, 62.16% and 59.90% was estimated in methanolic leaf, unripened
peel, and seed extract respectively. In methanolic seed extract, the cell viability at 500
µg/mL concentration was 25.29%, the extract lysed 74.70% MCF-7 cells with IC50 lysis value of
Conclusion: From the results obtained in the present study, it can be concluded that the leaf extracts
showed potential antidiabetic and anti-inflammatory activities. Peel extracts have high anti-oxidant
& anti-inflammatory activity and seed extracts showed higher level of MCF-7 cell line inhibition.