Abstract
Background: Electrochemical DNA sensor is a convenient and sensitive method for the detection of target ssDNA sequence. The developments of nanomaterials modified electrodes provide more opportunity for the enhancement of the analytical performances.
Objective: The aim of this paper is to construct a new electrochemical DNA sensor for the detection of Staphylococcus aureus nuc gene sequence with a chitosan-graphene (CTS-GR) and gold nanoparticles (AuNPs) modified electrode.
Methods: Electrodeposition was used to synthesize AuNPs directly on carbon ionic liquid electrode (CILE) to obtain AuNPs/CILE. Then CTS-GR nanocomposite was dropped on the surface of AuNPs/CILE to obtain CTS-GR/AuNPs/CILE. The nanocomposite on the electrode was suitable for immobilization of probe ssDNA sequence with electrostatic attraction. Hybridization took place with the complementary ssDNA sequences and assessed with methylene blue (MB) as electrochemical indicator.
Results: Due to the synergistic effects of CTS-GR and AuNPs, the modified electrode exhibited excellent electrochemical performances. The reduction peak currents of MB were in line with the concentration of the specific nuc gene sequences from 1.0×10-13 to 1.0×10-6 mol L-1 with a detection limit of 3.33×10-14 mol L-1 (3δ). This electrochemical DNA sensor displayed prominent selectivity to distinct different mismatched ssDNA sequences.
Conclusion: This electrochemical DNA biosensor exhibited properties such as higher sensitivity, wider linear range and lower detection limit, which were further applied to the PCR product detection with favorable results.
Keywords: Graphene, Gold nanoparticle, Electrochemical DNA sensor, Staphylococcus aureus nuc gene, Carbon ionic liquid electrode, chitosan-graphene (CTS-GR).
Graphical Abstract
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
Biogeochemistry: global environmental research and management
his issue focuses on the bio-pathways of trace elements (Ni, Co, Cr, Mn, Cd, Pb, Zn and REE) in soil, plant and atmospheric systems. Specific interest in the application of phytotechnologies that utilise hyperaccumulator plants (agro/phytomining). On the basis of understanding the migration and transformation of trace elements in ecosystem, ...read more
Related Journals
Anti-Cancer Agents in Medicinal Chemistry
Current Computer-Aided Drug Design
Current Bioactive Compounds
Combinatorial Chemistry & High Throughput Screening
Current Medicinal Chemistry
The Natural Products Journal
Current Catalysis
Current Chinese Chemistry
Current Chinese Science
Current Topics in Chemistry
Related Books
Botanicals and Natural Bioactives: Prevention and Treatment of Diseases
Biosurfactants: A Boon to Healthcare, Agriculture & Environmental Sustainability
Frontiers in Computational Chemistry
Advances in Dye Degradation
Biological and Medical Significance of Chemical Elements
Frontiers In Medicinal Chemistry
Advances in Organic Synthesis
Frontiers in Natural Product Chemistry
Recent Advances in Analytical Techniques
Advanced Catalysts Based on Metal-organic Frameworks (Part 2)
23
3
1
1