HPLC - MS / MS Determination of Fraxetin in Rat Plasma and its Application to a Pharmacokinetic Study

Title:HPLC - MS / MS Determination of Fraxetin in Rat Plasma and its Application to a Pharmacokinetic Study

Volume: 14 Issue: 4

Author(s): Lei Wang, Bingjing Zheng, Guangwen Luo, Lizhu Yang, Lingtian Wang, Zhen Hu*Zheng Xiang*

Affiliation:

• The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035,China

• School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035,China

Keywords: Fraxetin, HPLC-MS/MS, pharmacokinetic, rat, plasma, chromatography.

Abstract: Background: Fraxetin is mainly an active ingredient of Fraxini Cortex which is an important and traditional Chinese medicine for clinical applications, has heat-clearing, dispelling dampness, antitussive and anti-asthmatic effects. Fraxetin has a variety of pharmacological activities.

Method: The chromatographic separation was carried out on a Zorbax Eclipse XDB C18 column. The mobile phase consisted of acetonitrile (0.1 % formic acid) and ammonium acetate (0.1 % formic acid). Samples were prepared with protein precipitation.

Result: The method had good linearity within 10 - 2500 ng/mL. Both intra- and inter-assay precisions were acceptable and ≤ 8.5 %. Extraction recovery was 75.9 - 89.5 %, and the lower limit of quantification for fraxetin was 10 ng/mL. This proposed method was successfully used to study the pharmacokinetic and bioavailability of fraxetin after 5 and 25 mg/kg fraxetin were administered to rats via intravenous and oral routes, respectively. The half-life (t1/2) of fraxetin for i.v was (3.86 ± 0.65) h. The t1/2 of fraxetin for p.o was (4.41 ± 1.79) h. The bioavailability of fraxetin is 12.6%.

Conclusion: The results provided some useful information for the future development and clinical medication of fraxetin.

Cite this article as:

Wang Lei, Zheng Bingjing , Luo Guangwen, Yang Lizhu , Wang Lingtian, Hu Zhen*, Xiang Zheng*, HPLC - MS / MS Determination of Fraxetin in Rat Plasma and its Application to a Pharmacokinetic Study, Current Pharmaceutical Analysis 2018; 14 (4) . https://dx.doi.org/10.2174/1573412913666170525123017