HPLC - MS / MS Determination of Fraxetin in Rat Plasma and its Application to a Pharmacokinetic Study

Author(s): Lei Wang, Bingjing Zheng, Guangwen Luo, Lizhu Yang, Lingtian Wang, Zhen Hu*, Zheng Xiang*.

Journal Name: Current Pharmaceutical Analysis

Volume 14 , Issue 4 , 2018

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Abstract:

Background: Fraxetin is mainly an active ingredient of Fraxini Cortex which is an important and traditional Chinese medicine for clinical applications, has heat-clearing, dispelling dampness, antitussive and anti-asthmatic effects. Fraxetin has a variety of pharmacological activities.

Method: The chromatographic separation was carried out on a Zorbax Eclipse XDB C18 column. The mobile phase consisted of acetonitrile (0.1 % formic acid) and ammonium acetate (0.1 % formic acid). Samples were prepared with protein precipitation.

Result: The method had good linearity within 10 - 2500 ng/mL. Both intra- and inter-assay precisions were acceptable and ≤ 8.5 %. Extraction recovery was 75.9 - 89.5 %, and the lower limit of quantification for fraxetin was 10 ng/mL. This proposed method was successfully used to study the pharmacokinetic and bioavailability of fraxetin after 5 and 25 mg/kg fraxetin were administered to rats via intravenous and oral routes, respectively. The half-life (t1/2) of fraxetin for i.v was (3.86 ± 0.65) h. The t1/2 of fraxetin for p.o was (4.41 ± 1.79) h. The bioavailability of fraxetin is 12.6%.

Conclusion: The results provided some useful information for the future development and clinical medication of fraxetin.

Keywords: Fraxetin, HPLC-MS/MS, pharmacokinetic, rat, plasma, chromatography.

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Article Details

VOLUME: 14
ISSUE: 4
Year: 2018
Page: [349 - 354]
Pages: 6
DOI: 10.2174/1573412913666170525123017
Price: $65

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