Current Gene Therapy

Ignacio Anegon
Director INSERM UMR 1064-Center for Research in Transplantation and Immunology
CHU de Nantes. 30, boulevard
Nantes
France

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Gene Delivery Particle Engineering Strategies for Shape-dependent Targeting of Cells and Tissues

Author(s): Kristen L. Kozielski, Metin Sitti.

Abstract:

Background: Successful gene delivery requires overcoming both systemic and intracellular obstacles before the nucleic acid cargo can successfully reach its tissue and subcellular target location.

Materials & Methods: Non-viral mechanisms to enable targeting while avoiding off-target delivery have arisen via biological, chemical, and physical engineering strategies.

Discussion: Herein we will discuss the physical parameters in particle design that promote tissue- and cell-targeted delivery of genetic cargo. We will discuss systemic concerns, such as circulation, tissue localization, and clearance, as well as cell-scale obstacles, such as cellular uptake and nucleic acid packaging.

Conclusion: In particular, we will focus on engineering particle shape and size in order to enhance delivery and promote precise targeting. We will also address methods to program or change particle shape in situ using environmentally triggered cues.

Keywords: Gene delivery, Anisotropy, Nanoparticles, Self-assembly, Soft lithography, Shape-change.

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Article Details

VOLUME: 17
ISSUE: 2
Year: 2017
Page: [80 - 88]
Pages: 9
DOI: 10.2174/1566523217666170510161120
Price: $58