Background: Vibrational spectroscopy has shown the capacity to provide rapid, accurate
and relevant information on microorganism’s classification and identification. In fact Fourier Transform
Infrared Spectroscopy is a sensitive and effective methodology in food analysis, clinical diagnosis
and environmental evaluation, with successful identification at genus and species level.
Focus: The interest in this technique lies not only in the identification and classification of bacteria,
but there is also relevance in using FTIR to follow cellular responses to stress in unicellular models,
with particular interest in yeasts and so far results show FTIR is able to evaluate specific cellular
changes related to stress stimulus, mainly in protein region.
Prospect: This work gathered information that demonstrates that it is possible to overcome signal overlapping
and use Fourier Transform Infrared Spectroscopy and multivariate analysis tools to obtain biochemical
signatures of microorganisms and corresponding metabolome that can provide inexpensive
and highly specific alternatives to conventional typing methods. Also, it presents a spectrum representative
of a microbial cell and summarizes in a table the spectral assignments of main biological contributors.