Food-borne mycotoxins are now recognized as a major cause of food safety issue. Currently, a number
of immunochemical methods have been applied to mycotoxin analysis because of their high specificity, facilitate
sample preparation, and ease of use. These methods include widely applicable ELISAs, biosensors, and noninstrumental
immunoassays. As a rule, immunoassay is based on specific interaction between antigen and antibodies.
Aiming at improving the sensitivity and reliability of immunoassays for mycotoxins, researchers have
focused their efforts on developing natural (e.g. poly and monoclonal antibodies, antibody fragments, and nanobodies)
and artificial antibodies (e.g. aptamers, peptides, and molecular imprinted polymers). Furthermore, researchers
also found that the quality of mycotoxin-carrier conjugates is equally essential to the sensitivity and
reliability of immunoassays. Recently, various research has been conducted to develop substitutes for mycotoxin
conjugates include anti-idiotype antibodies, phage displayed peptides, and anti-idiotype nanobodies. This review
will focus on the general aspects of the development of antibody against mycotoxin and the main principles and
stages of the development of immunochemical methods for mycotoxins analysis.