Background: Electrochemistry technique has attracted significant attention over the past decade as a
potential candidate for the highly sensitive detection of analyte species.
Objective: The goal of the present work is to fabricate a novel electrochemical non-enzymatic glucose
sensor based on the nickel-copper/graphene oxide-modified glassy carbon electrode (Ni-
Method: A bare GCE was coated with graphene oxide, and then immersed in a deoxygenated solution
containing 50 mM NiCl2
and 50 mM CuCl2
for the electrodeposition at -0.6 V for 100 s. The modified
electrode's morphology and electrochemical performance were characterized using scanning electron
microscopy and cyclic voltammetry, respectively.
Results: The Ni-Cu/GO/GCE was found to exhibit a higher electrocatalytic activity for glucose oxidation
than did a nickel/graphene oxide-modified GCE in an alkaline solution. When used as a glucose
sensor, the electrode exhibited a sensitivity of 160.37 μA mM-1
for glucose oxidation with a linear
range from 15 μM to 1030 μM and a detection limit of 2 μM. The sensor was highly selective for
glucose even in the presence of common interfering species such as ascorbic acid, uric acid, and dopamine.
As a demonstration of its practicality, the Ni-Cu/GO/GCE was used to measure glucose in fetal
Conclusion: A non-enzymatic glucose sensor based on the Ni-Cu/GO/GCE with high sensitivity and
improved specificity was developed and characterized. Selective detection of glucose in a linear concentration
range of 15-1030 μM was obtained. The proposed method has potential to be applied for
accurate measurement of glucose level in real samples.