Background: Using Multi-Walled Carbon Nanotubes (MWCNTs) as a drug delivery system,
can avoid the need for solvents and preventing drug precipitation in aqueous solution. Soyasapogenol B
(SSB) acts as an important therapeutic agent owing to its numerous reported biological activities.
Hence, this work deals with preparation and characterization of SBB loaded onto functionalized
MWCNTs with tetraethyl orthosilicate (TEOS) and/or chitosan.
Method: SSB was immobilized onto functionalized MWCNTs using miniemulsion technique. Moreover,
niosomes were utilized to encapsulate the prepared systems. The formulations were analyzed by
Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscope (TEM) and particle
size distribution analysis. In vitro release profiles of loaded SSB particles were carried out and kinetics
of release were also studied. In vitro cytotoxicity of the prepared materials was examined and evaluated
by SRB assay using different human cell lines such as normal melanocytes (HFB4), and carcinoma
breast and liver (MCF7 and HepG2, respectively) in comparison with the standard doxorubicin.
Results: SSB loaded materials exhibited successful encapsulation in niosomes, resulting in sustainable
in drug release. Study of kinetics of release revealed presence of several complex factors affecting SSB
release. Mathematical processing of the in vitro release data showed that the release of SSB from
niosomal formulations obeyed more than one model. The second order and Higuchi’s models were the
most fitting models in case of presence of chitosan or TEOS, respectively. While, all formulations exhibited
low cytotoxic properties on all tested cell lines.
Conclusion: FTIR, particle size and TEM analysis confirmed that SSB was successfully loaded onto
functionalized MWCNTs. Moreover, the different niosome formulations based on functionalized
MWCNTs were prepared with sustainable SSB release in. The cytotoxicity could be minimized in case
of chitosan and TEOS functionalization.