Background: Diabetes mellitus (DM) is a major health problem with an increasing global
prevalence. It is usually associated with an imbalance between pro-oxidant mechanisms and antioxidant
defenses, contributing to oxidative-stress, and this leads to an increased susceptibility to endothelial
dysfunction, atherosclerosis, insulin-resistance and impaired-pancreatic β-cell function.
Objective: We have assessed the Prooxidant-antioxidant balance (PAB) and anti-hemolytic effect of
Thuja orientalis L using the PAB assay and the analysis of hematological markers.
Methods: The antioxidant and anti-hemolytic activity of Thuja orientalis was evaluated using the PAB
assay and the inhibition of RBC hemolysis using the hydrogen peroxide hemolysis test. The percentage
of anti-hemolysis was calculated from the ratio of the measurements (A-B)/B×100.
Results: Our results showed that the antioxidant effect of Thuja orientalis L. was greater in water than
in ethyl-acetate, ethanol and methanol extract, respectively. We also observed its anti-hemolytic effect,
which was higher in water, than in ethyl-acetate, methanol and ethanol extract, respectively. In particular
our data showed that the H2O2-induced RBC hemolysis was inhibited in a dose-dependent manner.
Conclusion: we demonstrated the antioxidant and anti-hemolytic effect of Thuja orientalis L. extracts in
human serum and RBC, showing its potential property of reducing free radicals supporting further investigations
to assess its functional role in larger samples size and in vivo models, as a potential antioxidant