Compositional Monosaccharide Analysis of Morus nigra Linn by HPLC and HPCE Quantitative Determination and Comparison of Polysaccharide from Morus nigra Linn by HPCE and HPLC

Author(s): Xiao-Li Ma*, Feng Feng Song, Hui Zhang, Xie Huan, Song Ya Li.

Journal Name: Current Pharmaceutical Analysis

Volume 13 , Issue 5 , 2017

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Abstract:

Background: Morus nigra Linn is not only treated as health food but also medicine in Chinese history.

Objective: Here, we have extracted and separated the heteroglycan to monosaccharides of the dry fruits.

Method: After heteroglycan being hydrolysised, then were we derivatived the monosaccharides with 1- phenyl-3-methyl-5-pyrazolone (PMP), and then subsequently HPCE and HPLC were used to separate. HPCE, which held an uncoated capillary (d=75µ m) and detected by PDA at 245 nm with borate buffer, the voltage was set at 15 kV and capillary temperature was 25°. There is Yilite column (Hypersil BDS C18 5µ m, 4.6mm× 250mm) with HPLC and detected by UV at 245 nm and capillary temperature 25°.The High Performance capillary electrophoresis method and High Performance Liquid Chromatography method were compared on monosaccharide composition and quantitative determination of polysaccharide from the black mulberry.

Results: Through several indicators including standard curve, precision, reproducibility, stability and recovery rate, the result of the two methods is basically consistent while they have complementary advantages.

Conclusion: Both methods are suitable for the determination of the black mulberry polysaccharide, but each has its own merits.

Keywords: Morus nigra Linn, polysaccharide, HPCE, HPLC, boric acid, linearity.

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Article Details

VOLUME: 13
ISSUE: 5
Year: 2017
Page: [433 - 437]
Pages: 5
DOI: 10.2174/1573412913666170330150807

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