Anti-cancer Effect of Cyanidin-3-glucoside from Mulberry via Caspase-3 Cleavage and DNA Fragmentation in vitro and in vivo

Author(s): Eugene Cho, Eun Y. Chung, Hye-Yeon Jang, On-Yu Hong, Hee S. Chae, Young-Ju Jeong, Sun-Young Kim, Byeong-Soo Kim, Dong J. Yoo, Jong-Suk Kim*, Kwang-Hyun Park*.

Journal Name: Anti-Cancer Agents in Medicinal Chemistry

Volume 17 , Issue 11 , 2017

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Abstract:

Background: Fruits of Morus alba L. (mulberry) have various bioactive compounds such as polyphenols and anthocyanins and used as a herbal medicine. However, the anti-cancer effects and molecular basis have not been elucidated.

Methods: We isolated the cyanidin-3-glucoside in various cultivar of mulberry by acidified-methanol extraction methods. This molecule were compared mass spectroscopic properties by LC-MS/MS and analyzed by 1H and 13C NMR. We examined the anti-cancer effect with molecular mechanisms of the cyanidin-3-glucoside on MDA-MB-453 human breast cancer cells and xenograft animal model.

Results: The treatment with the mulberry cyanidin-3-glucoside decreased cell viability in a dose-dependent manner with alteration of apoptotic protein contents, and DNA fragmentation, suggesting that cells undergo apoptosis. Supporting the observations, Treatment with the cyanidin-3-glucoside showed active apoptosis by caspase-3 cleavage and DNA fragmentation through Bcl-2 and Bax pathway. Indeed, cyanidin-3-glucoside inhibits tumor growth in MDA-MB-453 cells-inoculated nude mice. Tumor growth of xenograft nude mouse was significantly reduced compared to the control group by the cyanidin-3-glucoside.

Conclusion: The data demonstrate that cyanidin-3-glucoside isolated from mulberry induced apoptosis in breast cancer (MDA-MB-453) cells, and therefore, has a potential as an anti-cancer agent. These results show that mulberry cyanidin-3-glucoside inhibit the proliferation and growth in vitro and in vivo model and, indicating the inhibition of tumor progression.

Keywords: Cyanidin-3-glucoside, Morus alba L., mulberry, anti-cancer, caspase-3, DNA fragmentation, nude mice.

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Article Details

VOLUME: 17
ISSUE: 11
Year: 2017
Page: [1519 - 1525]
Pages: 7
DOI: 10.2174/1871520617666170327152026
Price: $58

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