Single Crystal X-Ray Diffraction in Structure Elucidation of Arborinine from Glycosmis pentaphylla

Author(s): Moon Moon Das , Dibakar Chandra Deka* , Ranjit Thakuria .

Journal Name: The Natural Products Journal

Volume 7 , Issue 3 , 2017

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Abstract:

Background: Arborinine is a naturally found alkaloid with various biological activities. It has been isolated from the leaf extract of Glycosmis pentaphylla, a traditionally used medicinal plant of North East of India. Single crystal X-ray diffraction on arborinine has never been reported earlier.

Objective: The main objective of the present study is to report the isolation and characterization of arborinine and its single crystal X-ray diffraction analysis.

Method: Arborinine was isolated from the leaf extract of Glycosmis pentaphylla by repeated column chromatography. Fine yellow needle-shaped crystals were recovered from a solution of purified arborinine in a mixture of ethyl acetate and petroleum ether (1:5). The purified compound is characterized by IR, NMR, HRMS and single crystal X-ray diffraction.

Results: The crystal structure was solved in orthorhombic Pbca space group with unit cell parameters a = 14.805(3) Å, b = 7.5342(15) Å, c = 23.620(5) Å, α= 90º, β = 90º, γ= 90º, V= 2634.7(9) Ǻ3, Ζ = 8, Dcalc = 1.438 Μgm-3 and F (000) = 1200. The molecules of arborinine with a planar acridine ring with flanking methoxy groups at 2- and 3- position display a 3D framework resulting from weak C-H•••O, π-π stacking and Van der Waal interactions.

Conclusion: Single crystal X-ray diffraction on arborinine is reported for the first time. High field NMR and high resolution mass are also reported for the first time on a sample of arborinine isolated from the plant Glycosmis pentaphylla.

Keywords: Arborinine, Glycosmis pentaphylla, single crystal X-ray diffraction, column chromatography, Van der Waal interactions, Rutaceae.

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Article Details

VOLUME: 7
ISSUE: 3
Year: 2017
Page: [224 - 230]
Pages: 7
DOI: 10.2174/2210315507666170323155126
Price: $58

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