Background and Objectives: Hydatid cyst is the larval stage of the tapeworm Echinococcus
granulosus. Hydatid cyst fluid, cyst membrane and Protoscolices, contain a complex mixture of antigens
that can induce immune responses in the host. Anti-cancer properties of Protoscolices and hydatid cyst fluid
has been shown. In order to identify antigens of hydatid cyst fluid that have anti-cancer effect, in this study
production of monoclonal antibodies against one of the hydatid cyst fluid band (40KDa) has been investigated.
There are many published patents about applications of monoclonal antibodies.
Methods: In this experimental study, 40KDa band of hydatid cyst fluid that has cross reaction with sera of
patients with breast cancer was used as antigen. A group of mice were immunized with this antigen, and then
their spleen cells were extracted and fused with SP2 cells. Monoclonal antibodies production was checked in
wells with signs of cell growth using ELISA and western blotting. The reaction of the produced monoclonal
antibodies with breast cancer cells was tested using flow cytometry method. Finally, effect of the monoclonal
antibodies on growth of breast cancer cells was investigated in vitro.
Results: The results of this study showed that in the first plate antibody against 40KDa was detected in several
wells. In the second plate monoclonal antibodies with high titer was detected in one well. The produced
monoclonal antibodies reacted with the surface of breast cancer cells. However, they had no significant effect
on growth of breast cancer cells in culture medium.
Conclusion: Monoclonal antibodies against hydatid cyst fluid 40KDa band were produced. These antibodies
reacted with the surface of breast cancer cells but had no significant effect on growth of these cells.