Additive Effect of MCP in Combination with Cefotaxime Against Staphylococcus aureus (E-pub Ahead of Print)
Roula M. Abdel-Massih.
Background: Pectin is a heterogeneous polysaccharide present in plants and citrus fruits.
It exhibits different beneficial biological activities. Conflicting reports exist about the antimicrobial
effect of pectin and its derivatives.
Objective: In this study, we investigate the antimicrobial effect of Modified Citrus Pectin (MCP)
against Staphylococcus aureus, a pathogen showing increasing rates of antimicrobial resistance
Methods: Forty-three clinical isolates of S. aureus were obtained from a hospital in North Lebanon.
Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal Concentrations (MBCs)
were determined using MCP after determining its optimum pH activity. The combination between
MCP and cefotaxime was then investigated for S. aureus isolates using the checkerboard technique.
Results and Discussion: The optimum pH for the activity of MCP was 6.0. MIC and MBC values
against S. aureus ranged between 0.39–50 μg/μl and 3.13–50 μg/μl, respectively. These values are
promising for using MCP in the inhibition of some S. aureus isolates at relatively low concentrations.
Combination experiments showed an additive effect in most S. aureus strains between MCP
and cefotaxime, and a synergistic effect in two strains. These preliminary findings open the way for
further investigation into the therapeutic potential of MCP in the treatment of S. aureus infections.
Conclusions: MCP demonstrates in vitro antimicrobial activity alone and in combination with cefotaxime
against S. aureus.
Keywords: Staphylococcus aureus, Modified Citrus Pectin, Checkerboard, Antibiotic Resistance, MRSA, Combination therapy.
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