Detection of Squamous Cell Carcinoma Antigen with Micro- and Nanogap Interdigitated Electrodes and Gold Nanoparticles

Author(s): Thangavel Lakshmipriya*, Uda Hashim*, C. B. Gopinath.

Journal Name: Micro and Nanosystems

Volume 9 , Issue 1 , 2017

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Abstract:

Background: Biosensor detects the binding event of biologically relevant molecules, which are analyte and ligand. Showing improved sensitivity is the key and primary characteristic of a high-performance biosensor, which displays lower abundance of an analyte. Here, we preferred the silicon substrate on impedance sensor to demonstrate the improvement in the sensitivity with different band-gaps.

Methods: we have fabricated two band-gaps on the sensing substrate with the sizes of micrometer and nanometer. These gaps were measured to be 85.5 ± 5 µm and 125 ± 3 nm, respectively. Squamous cell carcinoma (SCC) antibody and antigen interaction for the cancer detection was demonstrated on these gaps.

Results: It was found that nano-gap is having higher sensitivity than micro-gap. Further, gold nanoparticle conjugated SCC antigen was found to show the improvement with the micro-gap.

Conclusion: This research has evidenced that lesser band-gap (nano) exhibits a good sensitivity for the detection of SCC antigen rather than micro-gap. Gold nanoparticle conjugation with antigen is suitable for microgaps, whereas no significant improvement with nano-gaps was observed.

Keywords: Micro-gap, nano-gap, biosensor, SCC-antigen, gold nanoparticles, squamous cell carcinoma, nanometer.

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Article Details

VOLUME: 9
ISSUE: 1
Year: 2017
Page: [2 - 6]
Pages: 5
DOI: 10.2174/1876402909666170301101312
Price: $58

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