Principal Physicochemical Methods Used to Characterize Dendrimer Molecule Complexes Used as Genetic Therapy Agents, Nanovaccines or Drug Carriers

Author(s): Rodríguez Fonseca Rolando Alberto , Rodrigues Joao , Muñoz-Fernández María de los Angeles , Martínez Muñoz Alberto , Fragoso Vázquez Manuel Jonathan* , Correa Basurto José .

Journal Name: Current Pharmaceutical Design

Volume 23 , Issue 21 , 2017

Abstract:

Nanomedicine is the application of nanotechnology to medicine. This field is related to the study of nanodevices and nanomaterials applied to various medical uses, such as in improving the pharmacological properties of different molecules. Dendrimers are synthetic nanoparticles whose physicochemical properties vary according to their chemical structure. These molecules have been extensively investigated as drug nanocarriers to improve drug solubility and as sustained-release systems. New therapies such as gene therapy and the development of nanovaccines can be improved by the use of dendrimers. The biophysical and physicochemical characterization of nucleic acid/peptide-dendrimer complexes is crucial to identify their functional properties prior to biological evaluation. In that sense, it is necessary to first identify whether the peptide-dendrimer or nucleic aciddendrimer complexes can be formed and whether the complex can dissociate under the appropriate conditions at the target cells. In addition, biophysical and physicochemical characterization is required to determine how long the complexes remain stable, what proportion of peptide or nucleic acid is required to form the complex or saturate the dendrimer, and the size of the complex formed. In this review, we present the latest information on characterization systems for dendrimer-nucleic acid, dendrimer-peptide and dendrimer-drug complexes with several biotechnological and pharmacological applications.

Keywords: Nanovaccines, gene therapy, dendrimers, peptides, nucleic acids, functional properties characterization.

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Article Details

VOLUME: 23
ISSUE: 21
Year: 2017
Page: [3076 - 3083]
Pages: 8
DOI: 10.2174/1381612823666170220164535
Price: $58

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