Nanomedicine is the application of nanotechnology to medicine. This field is related to the study of
nanodevices and nanomaterials applied to various medical uses, such as in improving the pharmacological properties
of different molecules. Dendrimers are synthetic nanoparticles whose physicochemical properties vary
according to their chemical structure. These molecules have been extensively investigated as drug nanocarriers to
improve drug solubility and as sustained-release systems. New therapies such as gene therapy and the development
of nanovaccines can be improved by the use of dendrimers. The biophysical and physicochemical characterization
of nucleic acid/peptide-dendrimer complexes is crucial to identify their functional properties prior to
biological evaluation. In that sense, it is necessary to first identify whether the peptide-dendrimer or nucleic aciddendrimer
complexes can be formed and whether the complex can dissociate under the appropriate conditions at
the target cells. In addition, biophysical and physicochemical characterization is required to determine how long
the complexes remain stable, what proportion of peptide or nucleic acid is required to form the complex or saturate
the dendrimer, and the size of the complex formed. In this review, we present the latest information on characterization
systems for dendrimer-nucleic acid, dendrimer-peptide and dendrimer-drug complexes with several
biotechnological and pharmacological applications.
Keywords: Nanovaccines, gene therapy, dendrimers, peptides, nucleic acids, functional properties characterization.
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