Advances in Modern Medicine

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Advances in Modern Medicine introduces recent advanced medical practices performed at the Kure Medical Center and Chugoku Cancer Center (KMCCCC) - one of the leading hospitals in Japan - to those ...
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Quality Assurance of Immunohistochemistry for Breast Cancers using a Whole-slide Imaging System, Specified Software and Cell Lines

Pp. 477-480 (4)

Miho Yoshida-Tanaka, Junichi Sakane, Yoshiko Kimura, Yoshimi Shitakubo, Kazuya Kuraoka and Kiyomi Taniyama

Abstract

An auto-stainer (AS), whole-slide imaging system (WSI), and computer software were used as quality assurance tools for breast cancer immunohistochemistry (IHC).

Cell lines, such as MCF-7 for estrogen receptors and progesterone receptors, and SKBR- 3 for Topo2a, were co-stained with samples as IHC control.

Combinatory usage of AS, WSI, computer software and cell lines can reduce deviation in each IHC step and is beneficial for IHC quality assurance.

Keywords:

Auto-analysis, Breast cancer, Immunohistochemistry, Quality assurance, Whole slide image.

Affiliation:

National Hospital Organization, Kure Medical Center and Cancer Center, 3-1, Aoyama-cho, Kure 737-0023, Japan.