Title:CXCR4-targeted Nanoparticles Reduce Cell Viability, Induce Apoptosis and Inhibit SDF-1α Induced BT-549-Luc Cell Migration In Vitro
VOLUME: 14 ISSUE: 8
Author(s):Chuda Chittasupho*, Prartana Kewsuwan and Takashi Murakami
Affiliation:Department of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot University, Ongkarak, Nakhonnayok, 26120, Thailand Institute of Nuclear Technology, Ongkarak, Nakhonnayok, 26120, Department of Microbiology, Saitama Medical University, Iruma-gun, Saitama 350-0495
Keywords:Breast cancer, CXCR4, LFC131, metastasis, nanoparticle, PLGA.
Abstract:Background: CXCR4 possesses a critical role in several intracellular events such as chemotaxis,
invasion and adhesion, which are associated with metastasis of cancer cell.
Objective: In this study, CXCR4 targeted polymeric nanoparticle was developed for delivering cytotoxic
drug and blocking the chemokine induced migration of cells expressing CXCR4.
Method: A peptide which was a linear form of CXCR4 antagonist (LFC131) was attached to PLGA
nanoparticles (LFC131-NPs) and PLGA nanoparticles encapsulating DOX (LFC131-DOX-NPs). The
cellular binding and internalization of LFC131-DOX-NPs were investigated.
Results: The binding and internalization of LFC131-DOX-NPs were higher and more rapidly compared
to unconjugated NPs. LFC131-NPs blocked SDF-1α induced migration of BT-549-Luc cells.
MTT assays demonstrated that LFC131-NPs and LFC131-DOX-NPs decreased cell viability in a dose
dependent manner in 24, 72 and 120 h incubation.
Conclusion: A treatment concept of blocking breast cancer cell migration from interaction with SDF-
1α by using LFC131-NPs and then attacking breast cancer cells with doxorubicin might increase the
efficacy of current breast cancer treatment.
