Background: pyrazolo[3',4':4,5]pyrimido[1,6-b][1,2,4]-triazines have been found to possess a wide
range of medical activities.
Objective: we interested to synthesis a new series of pyrazolo[3',4':4,5]pyrimido[1,6-b][1,2,4]triazines via the
reaction of 1,3-diphenyl-4-hydrazino-pyrazolo[3,4-d]pyrimidine with ethyl aroyl pyrovates and invistigate thier
antimicrobial activity. Not only the biological activity is our aim but also, to elucidate the actual tautomeric structure
of the products.
Method: A new series of pyrazolo[3',4':4,5]pyrimido[1,6-b][1,2,4]triazine derivatives was prepared via condensation
of ethyl aroylpyruvates with 1,3-diphenyl-4-hydrazino-pyrazolo[3,4-d]pyrimidine. Also, treatment of
these compounds with hydrazine hydrate afforded a new series of 1,3-diphenyl-4-(7-aryl-2,6-dihydropyridazino[
Results: The mechanism of the reaction was discussed and proved to proceed via Dimroth rearrangement. The
structure of all the newly synthesized compounds was established on the basis of spectral data and elemental
analysis. The antimicrobial activity of the products was screened and some derivatives showed promising activities
against the used bacteria and fungi species.
Conclusion: We success in synthesis of a new series of bioactive pyrazolo[3',4':4,5]pyrimido[1,6-b][1,2,4]triazines
and elucidation of their actual structure.