Antimicrobial, Gastroprotective and Healing Effect of the Hydroalcoholic Extract of Astronium fraxinifolium

Author(s): Anita Oliveira Brito Pereira Bezerra Martins, Ana Luiza Albuquerque Siebra, Cicera Datiane de Morais Oliveira, Gerlania Oliveira Leite, Henrique Douglas Melo Coutinho, Margareth Linde Athayde, Aline Augusti Bolignon, Jacqueline Cosmo Andrade, Larissa Rolim Oliveira, Marta Regina Kerntopf, Roseli Barbosa, Francisco Rafael Alves Santana Cesario, Thales Silva Coutinho, Lucindo Jose Quintans-Junior, Irwin Rose Alencar de Menezes*.

Journal Name: Letters in Drug Design & Discovery

Volume 15 , Issue 3 , 2018

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Abstract:

Introduction: Astronium fraxinifolium (Anarcadiaceae) is used in folk medicine in inflammation diseases. This study evaluates the chemical profile as well as antioxidant, antimicrobial, gastroprotective and wound healing activities of the hydroalcoholic extract of Astronium fraxinifolium bark (HAAF).

Material and Methods: Phytochemical screening and high-performance liquid chromatography (HPLC). The antioxidant activity was determined by DPPH (2,2- diphenyl, 1-picrihidrazila) and FRAP (ferric fraction reductive) methods. The gastroprotective activity was evaluated against HAAF to classical models of acute gastric: absolute ethanol, acidified ethanol and Indomethacin (50, 100 and 200mg/kg/orally) and physical barrier mechanism (50mg/kg/orally, intraperitoneally), used Swiss mice (Mus musculus). The healing activity of HAAF was identified from tissue shrinkage rate in the control. The antimicrobial activity was observed from the Minimum Inhibitory Concentration method (MIC) and modulation associated with antibiotics (amikacin, gentamicin, benzylpenicilin, imipinem + cilastatin sodium, cephalothin, oxalacina and ampicillin) and antifungals (amphotericin B, nystatin, mebendazole and benzoilmetronidazol).

Results: Phytochemical prospection of Chromatography (HPLC) quantified gallic acid (4.25 %), chlorogenic acid (2.11%), caffeic acid (0.73%), rutin (0.08 %) and quercetin (0.29%). Measurement of total flavonoids (0.034 g of quercetin) and phenols (0.538 g of gallic acid) gram of extract showed significant amount. HAAF showed significant antioxidant potential in DPPH and FRAP assays. The MIC did not show clinically relevant activity (≥1024 µg/mL). HAAF displayed gastroprotective different doses (50, 100 and 200 mg/Kg) and Healing activity (100 mg/g).

Conclusion: The results obtained in this study provide biological evidence of the antioxidant potential, gastroprotective and wound healing effect of the species.

Keywords: Astronium fraxinifolium, Antioxidant, Modulatory effect, antifungic effect, antibacterial activity, gastroprotection, healing.

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Article Details

VOLUME: 15
ISSUE: 3
Year: 2018
Page: [325 - 334]
Pages: 10
DOI: 10.2174/1570180814666170213160114
Price: $58

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