In vitro Evaluation of Biological Effects of Metallic Compounds with Quinolines

Author(s): Fernando L.A. Fonseca*, Taiana C. Caetano, Carolina Z. P. Mecca, Roberta S.S. Yamaguchi, Leandro R. Bissoli, Beatriz da Costa A. Alves, Izilda A. Bagatin.

Journal Name: Current Enzyme Inhibition

Volume 14 , Issue 1 , 2018

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Abstract:

Introduction: Modified quinolines have been used as metal chelators, and they have drawn the attention of researchers due to the possible biological approaches with a broad scope of application. This study aims to evaluate the in vitro biological effects of seven quinolinic compounds in RAW macrophage culture, blood cells and in Candida albicans fungus.

Methods: Cell viability and proliferation of macrophages were evaluated using trypan blue and MTT staining assays. Gene expression was analyzed through RT-qPCR. Hematological analysis was carried out using flow cytometry and coagulogram tests. The analysis of Candida albicans strains was performed through the Minimum Inhibitory Concentration test (MIC).

Results: The compounds did not significantly interfere with cell viability and proliferation of macrophages; however, compounds 6 and 7 increased the expression of both TNF-α and TGF-β1. Compound 3, in turn, only increased the expression of TNF-α and compound 4 increased TGF-β1 expression.

Conclusion: There were significant alterations in blood cellularity and in human coagulation cascade. Nevertheless, no fungicidal or fungistatic effect could be observed regarding Candida albicans.

Keywords: Blood cells, Candida albicans, coagulation cascade, metallic compounds, quinoline, RAW macrophages.

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Article Details

VOLUME: 14
ISSUE: 1
Year: 2018
Page: [18 - 25]
Pages: 8
DOI: 10.2174/1573408013666170213152751

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