Background: Docetaxel is a potent anticancer agent used in various cancer types. Attempts
are being made to improve docetaxel's pharmacokinetics because desirable modifications in disposition
and biodistribution characteristics could enhance the therapeutic outcomes of the drug. Quantitative
analysis of docetaxel in the body is important to understand modifications necessary to improve
the drug's biodistribution and pharmacokinetics. Majority of studies have applied HPLC coupled with
mass spectrometry (LC-MS) or HPLC alone to quantitatively analyse docetaxel in various matrices.
Long separation times, many resources, and additional techniques are usually required to establish a
proper liquid chromatographic (LC) method.
Objective: Development and validation of a simple and rapid mass spectrometric method (ESIMS/
MS) for quantification of docetaxel in mouse biological matrices were the objective of the study.
Methods: Docetaxel was extracted from mouse serum and liver using a liquid-liquid extraction with
tert-butyl methyl ether. Samples were direct-injected to the instrument using 0.1% formic acid in
methanol as the mobile phase (isocratic elution). Method development and validation were performed
according to FDA guidelines. Detection was done by a Hybrid Triple Quadrupole/Linear Ion trap instrument
through positive ESI source and MRM mode.
Result: Docetaxel retention-time was about 0.6 min while the total run-time was 2 min. The method
was linear, sensitive, and specific with an acceptable level of precision and accuracy and was successfully
applied for rapid quantitative analysis of docetaxel in mouse biological matrices.
Conclusion: The validated method allows simple and fast analysis of docetaxel in biological samples
for pharmacokinetic and biodistribution studies.