Validated Spectrodensitometric Method for Simultaneous Estimation of Sofosbuvir, Ribavirin and Saxagliptin in their Pure and Pharmaceutical Dosage Forms

Author(s): Salwa R. El-Shaboury, Samia M. El-Gizawy, Noha N. Atia, Mohammad Nabil Abo-Zeid*.

Journal Name: Current Pharmaceutical Analysis

Volume 14 , Issue 3 , 2018

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Graphical Abstract:


Background: A simple, sensitive and precise spectrodensitometric method has been developed and validated for simultaneous determination of sofosbuvir, ribavirin and saxagliptin in their pure and pharmaceutical dosage forms.

Methods: The method employed TLC plates precoated with silica gel G 60 F254 as the stationary phase. The mobile phase consisting of acetonitrile-water (80:20, v/v) was used to give compact bands for all the studied drugs at 228 nm. They were resolved with retardation factor (Rf) values of 0.71, 0.36 and 0.21 for sofosbuvir, ribavirin and saxagliptin respectively.

Results: The linearity ranges were 400 – 10000 ng/band for all the studied drugs. Limits of detection were 124.78, 124.31 and 128.29 and limits of quantitation were 378.13, 376.71, 388.77 ng/band for sofosbuvir, ribavirin and saxagliptin, respectively. Correlation coefficient (r) values were 0.9993, 0.9995 and 0.9991 for sofosbuvir, ribavirin and saxagliptin, respectively.

Conclusion: The method was validated according to ICH guidelines and has been successfully applied for the determination of the studied drugs in their pure and dosage forms without any interference from the commonly encountered excipients.

Keywords: TLC-spectrodensitometry, sofosbuvir, ribavirin, saxagliptin, pharmaceutical preparations, HCV.

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Article Details

Year: 2018
Page: [212 - 218]
Pages: 7
DOI: 10.2174/1573412913666170210151615
Price: $65

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PDF: 13