Background: Aging is a risk factor of age-related diseases. With the increasing number
of patients, serious consequences, and heavy economic burden, demands for drugs used to treat agerelated
diseases have increased. As such, anti-aging substances should be isolated to develop drugs
for the prevention and treatment of age-related diseases.
Method: In this study, a methanol extract of immature Arabidopsis thaliana seeds with coat was
separated by using a K6001 yeast bioassay system. In order to investigate the action mechanism,
four mutants, namely, Δuth1, Δskn7, Δsod1, and Δsod2 with K6001 background were employed and
the anti-oxidative stress assay was performed.
Results: One new anti-aging lysophosphatidic acid (LPA) was obtained, and its structural and stereochemical
characteristics were elucidated through spectroscopy and chemical derivatization. LPA
can extend the replicative lifespan of K6001 at 10 and 30 µM (p < 0.001 and p < 0.01, respectively).
This finding was comparable to the effect of resveratrol, a well-known anti-aging substance. However,
the anti-aging activity of the compound on the four mutants was diminished. In the antioxidative
stress assay, LPA improved the oxidative resistance of yeast cells.
Conclusion: The new LPA may exert its anti-aging effect by improving the anti-oxidative ability of
yeast cells. The genes of UTH1, SKN7, and SOD may also be involved in the action.