Background and Objective: Nanoparticles (NPs) have been recognized as important drug delivery
agents in several instances including the delivery of antibiotics against intracellular pathogenic
microorganisms. In this work the activity of self-assembled nanoparticles (NPs) of clarithromycin
(CLA), cationic lipid and polyelectrolytes against important mycobacteria such as Mycobacterium abscessus
Methods: NPs from injection of a CLA/cationic lipid ethanol solution in aqueous carboxymethylcellulose
(CMC) solution followed or not by the addition of poly (diallyldimethylammonium chloride)
(PDDA) to the anionic CLA/cationic lipid/CMC NPs were characterized by dynamic light scattering for
sizing, zeta-potential and polydispersity, scanning electron microscopy (SEM) for morphology, colloidal
stability along time from sizing and macroscopic observation, inhibitory activity against mycobacteria
from determination of minimal inhibitory concentrations (MIC), toxicity against macrophages in culture
from determinations of macrophages viability and inhibition of mycobacterial growth in biofilms.
Results: Anionic CLA/cationic lipid/CMC NPs incorporated higher percentage of CLA than the cationic
CLA/cationic lipid/CMC/PDDA NPs and showed higher antimicrobial activity against M. abscessus and
lower toxicity to macrophages than the cationic NPs.
Conclusion: These findings opened the possibility of extending the incorporation of other lipophilic antibiotics
or drugs into the here described self-assembled NPs and showed NPs adequacy to deliver antibiotics
and drugs intracellularly for sustained release inside cells and activity against biofilms growth.