Development of In Vitro Co-Culture Model in Anti-Cancer Drug Development Cascade
Frances M. Richards.
Background: Tumour microenvironment is recognized as a major determinant of intrinsic
resistance to anticancer therapies. In solid tumour types, such as breast cancer, lung cancer and
pancreatic cancer, stromal components provide a fibrotic niche, which promotes stemness, EMT,
chemo- and radioresistance of tumour. However, this microenvironment is not recapitulated in the
conventional cell monoculture or xenografts, hence these in vitro and in vivo preclinical models are
unlikely to be predictive of clinical response; which might attribute to the poor predictively of these
preclinical drug-screening models.
Conclusion: In this review, we summarized recently developed co-culture platforms in various
tumour types that incorporate different stromal cell types and/or extracellular matrix (ECM), in the
context of investigating potential mechanisms of stroma-mediated chemoresistance and evaluating
novel agents and combinations. Some of these platforms will have great utility in the assessment of
novel drug combinations and mechanistic understanding of the tumor-stroma interactions.
Keywords: Tumour microenvironment, drug development, tumour-stroma crosstalk, co-culture, cancer, anti-cancer drug.
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