Background: Due to the importance of 1,3,4-thiadiazoles and phthalimides in anticancer
agents, a novel series of 1,3,4-thiadiazole-phthalimide system have been synthesized and evaluated in
vitro against HT-29 and MCF-7 human cancer cell lines.
Methods: The target compounds were prepared through four-step reaction and their cytotoxicities
were evaluated by MTT assay.
Results: The results showed that 4-nitrobenzoyl moiety containing derivatives are the most potent
ones. The morphological evaluation also indicated that these compounds are apoptotic inducers.
Conclusion: 4-Nitro-N-(5-((3-(1,3-dioxoisoindolin-2-yl)propyl)thio)-1,3,4-thiadiazol-2-yl)benzamide (8m)
exhibits the best inhibitory effect against HT-29 and MCF-7 cell lines with IC50 values of 23.83 and
27.21 μM, respectively.