Aim and objective: Due to the common ethnopharmacological used or scientifically
examined biochemical properties, Elaeagnaceae family, Elaeagnus umbellata Thunb (EU, Güz yemişi)
was worth investigating.
Materials and methods: In this investigation, we revealed antioxidant, antiproliferative and enzyme
inhibition activities of the water, methanol, ethanol, acetone, ethyl acetate and hexane extracts of EU as
well as the contents of their phenolic, flavonoid, anthocyanin, ascorbic acid, lycopene and β-carotene.
The antioxidant activity was screened by total antioxidant (phosphomolybdenum assay), inhibition of
linoleic acid peroxidation, reducing power, deoxyribose assay, H2O2 scavenging and metal chelating
activities of the samples were tested in vitro. Additionally, the scavenging activities of the extracts were
determined against 1,1-diphenyl-2-picrylhydrazyl (DPPH˙), 2,2-azino-bis(3-ethylbenzothiazloine-6-
sulfonicacid (ABTS˙+), superoxide anion and peroxide radicals. The samples were determined for their
inhibitory activities against urease, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). In
vitro, antiproliferative activities of six different extracts were tested using the xCELLigence system
against HeLa and HT29 cell lines.
Results: The antioxidant activities of the extracts were found higher than standard antioxidants. The
water extracts of fruit and leaf showed the best antioxidant activity. In inhibition assays of urease,
AChE and BuChE, all extracts exhibited remarkable inhibition potential. Ethyl acetate extracts,
especially, showed better inhibition capacity. It was found that the antioxidant activities of the extracts
presented consistently with their chemical contents. The antiproliferative activities of leaf extracts were
more effective than the fruit extracts. The chromatographic methods were applied to the different
solvents to analyses phenolic secondery metabolites. It was found that fumaric acid, 4-hydroxybenzoic
acid, rutin and quercetin-3-β-D-glucoside, neohesperidin, hesperidin determined to have higher
contents all the extracts.
Conclusion: EU can be suggested as a potential natural source of antioxidants appropriate for
utilization in nutritional/pharmaceutical fields.