Background: Melanoma is known as the most dangerous form of skin cancer; whereas the
malignant choroidal melanoma is an orphan disease known as the most common primary intraocular
malignancy in adults. Literature suggests that the consumption of garlic and mistletoe leads to a reduced
risk of developing cancer.
Objective: The aim of this study was the obtaining and the characterization of polymer structures containing
mistletoe or garlic extract.
Methods: The structures were obtained in a polyaddition process combined with a spontaneous emulsification;
they were characterized by pH, size, Zeta potential and DSC measurements, evaluation of
encapsulation efficacy, penetrability through membranes and in vitro cytotoxicity tests.
Results: The microstructures present sizes between 1.05 and 2.60 µm and Zeta potentials between -7
and +36 mV. A good encapsulation was observed on different evaluations (88-92%). It was determined
that approx. 30% of polymer microstructures containing vegetal extracts pass through an artificial
membrane in 4 days. An in vitro cytotoxicity test revealed that these products are safe for administration.
The analysis of antitumor efficacy indicates that garlic extracts have important effects after 48
and 72 hours on A375 cells; however, polymer microstructures with herbal extracts did not reveal antiproliferative
activities on A375 cells because these polymer structures present a slow degradation.
Conclusion: Sterile eye drops solutions based on polymer microstructures containing garlic or mistletoe
extracts were obtained; the sample based on garlic extracts may be used in the pharmaceutical field
as drug carrier with an antiproliferative effect which occurs after a prolong period.